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Rabbit antibody to beta NGF: affinity purified

$227.00USD


Catalogue No. R-174-20
Description
This product is no longer available. Please see below for our recommended alternative products.
Replaced by R-093-500 IgG
Batch No. See product label
Unit size 20 g
Antigen Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Other Names Beta-nerve growth factor; Ngfb
Accession NGF_MOUSE
Produced in Rabbit
Purity Affinity purified
Applications IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 g/ml is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 g/ml (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown.
Cross-reactivity This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
Blast it If you would like to see the shared identity between different species or other proteins follow the link in Accession field, select then the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
Form Lyophilised
Reconstitution Reconstitute in 20 l of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 4C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Expiry Date 12 months after purchase
Specific References 1. Zhang H.T. et al (2008) Temporal changes in the level of neurotrophins in the spinal cord and associated precentral gyrus following spinal hemisection in adult Rhesus monkeys J Chem Neuroanat. 2008 Dec;36(3-4):138-43.
2. Zhang H.T. et al (2007) Immunohistochemical distribution of NGF, BDNF, NT-3, and NT-4 in adult rhesus monkey brain J Histochem Cytochem. 2007 Jan;55(1):1-19.
References 1. Ebendal, T. et al (1989) J Neurosci Res 22, 223-240.
2. Zhou, X. F. et al (1994) J Neurosci Methods 54, 95-102.
3. Angeletti, P. U. et al (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-75.
4. Hesse K. et al. (1997) Neurosci Lett. Aug 8;231(2):83-6.
5. Miao J et al. (2012) Neurosci Res. Dec;74(3-4):269-76.
Images (click to zoom)
Rabbit antibody to beta NGF: affinity purified Immunohistochemical staining of nerve growth factor (NGF) in rat cervical ganglion using rabbit polyclonal to native mouse NGF, catalogue number R-174-20.

PDF Data Sheet

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MSDS

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