| Catalogue No. |
R-1824-100 |
| Description |
May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439). Ref: uniprot.org. |
| Batch No. |
See product label. |
| Unit size |
100 ug |
| Antigen |
E-coli derived human CD133/PROM1 recombinant protein (position: P531-H865) has been used as the immunogen. Human CD133 shares 61% amino acid sequence identity with mouse CD133. |
| Antibody Type |
Polyclonal |
| Isotype |
IgG |
| Other Names |
Prominin-1, Prominin-like protein 1, PROM1, PROML1, Antigen AC133, MSTP061 |
| Accession |
O43490 PROM1_HUMAN |
| Produced in |
Rabbit |
| Purity |
Affinity purified |
| Applications |
Western Blot (0.1-0.5 ug/mL): tested in MCF-7 whole cell lysate. Immunohistochemistry: paraffin-embedded section (0.5-1.0 ug/mL): tested in human tonsil tissue. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
This antibody is specific for CD133 as demonstrated by western blotting. |
| Species Against |
Human |
| Antibody Against |
CD133/PROM1 |
| Cross-reactivity |
Human, other species not tested yet. |
| Form |
Lyophilized. |
| Reconstitution |
Spin vial briefly before opening. Reconstitute in 100 uL sterile, ultrapure water to prepare 1 mg/mL solution. Centrifuge to remove any insoluble material. |
| Storage |
Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. |
| Expiry Date |
12 months after purchase if unopened. |
