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Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum

$297.00USD


Catalogue No. R-142-100
Description FUNCTION: Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. SUBUNIT: Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ULK1. SUBCELLULAR LOCATION: Golgi apparatus. TISSUE SPECIFICITY: Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine. SIMILARITY: Belongs to the MAP1 LC3 family. ESTIMATED MOLECULAR WEIGHT: 13.667kDa.
Batch No. See product label
Unit size 100 l
Antigen A synthetic peptide (CVESAKIRAKYP) corresponding to the N-terminal of human GABARAP L2 (GABARAPL2) protein has been used as the immunogen. The sequence is homologous with mouse and rat form of GABARAP L2 (GABARAPL2).
Other Names Gamma-aminobutyric acid receptor-associated protein-like 2; GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; GEF-2; General protein transport factor p16; MAP1 light chain 3-related protein; GABARAPL2; FLC3A; GEF2;
Accession GBRL2_HUMAN
GBRL2_MOUSE
GBRL2_RAT
Produced in Rabbit
Purity Whole serum
Applications IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity IHC, WB and ELISA confirmed the specificity for GABARAP L2 (GABARAPL2). A 14kDa band, that corresponds to the molecular weight of GABARAPL2, is detected via western blot analysis.
Cross-reactivity Human, rat. Other species not yet tested.
Form Lyophilised
Reconstitution Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 4C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date Six months after purchase
References 1. Okazaki N. et al. Brain Res. Mol. Brain Res. 85:1-12(2000)
2. Xin Y. et al. Genomics 74:408-413(2001)
3. The MGC Project Team. Genome Res. 14:2121-2127(2004)
Images (click to zoom)
Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum Western blot under reducing conditions on 293 cell lysate using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1:100.
Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum A. Confocal microscopy on immunofluorescently detected MPO (in green) in cytospin-isolated human white blood cells counter-stained with Hoechst Dye. Here, the merged picture is presented.
Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum B. Confocal microscopy on immunofluorescently detected GABARAP L2 (GABARAPL2) in cytospin-isolated human white blood cells using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were stained for GABARAP L2 (GABARAPL2) appearing in red counter-stained with Hoechst DyeHere. The merged picture is presented.
Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum C is the merged picture of Figures A & B. Confocal microscopy on immunofluorescently detected GABARAP L2 (GABARAPL2) in cytospin-isolated human white blood cells using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for GABARAP L2 (GABARAPL2) appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were counter stained with Hoechst Dye (blue colour).
Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum Western blot under reducing conditions on Odora (an Olfactory cell line) cell lysate using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1:100.
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