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Rabbit antibody to Glial Fibrillary Acidic Protein (GFAP): IgG

$297.00USD


Catalogue No. R-164-250
Description
This product is no longer available. Please see below for our recommended alternative products.
Replaced by R-1374-50
Batch No. See product label
Unit size 250 g
Antigen Native GFAP extracted from rat spinal cord
Other Names Glial fibrillary acidic protein; astrocyte
Accession GFAP_HUMAN
GFAP_MOUSE
GFAP_RAT
GFAP_BOVINE
Produced in Rabbit
Purity Protein G purified IgG
Applications IHC. A dilution of 1:500-1:1000 is recommended. This antiserum works superbly for staining of paraffin-embedded tissue sections fixed in formalin, frozen sections and cell cytospins. Proteinase-K pre-treatment of tissues has been shown to produce most specific pattern of staining with negligible background. Occasionally, antigen retrieval by microwave (heat retrieval of the epitope) can produce some non-specific staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity This antibody is specific for GFAP shown by IHC and ELISA.
Cross-reactivity Human, mouse, rat and cow and expected to react to GFAP protein of origins of cat, dog and sheep
Blast it If you would like to see the shared identity between different species or other proteins follow the link in Accession field, select then the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
Form Lyophilised
Reconstitution Reconstitute in 250 l of sterile water. This will result in a concentration of 1 mg/ ml. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 4C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Expiry Date 12 months after purchase
References 1. Reeves S.A, et al. Proc. Natl. Acad. Sci. U.S.A. 86:5178-5182(1989).
2. Brenner M, et al. Brain Res. Mol. Brain Res. 7:277-286(1990).
2. Isaacs A, et al. Genomics 51:152-154(1998).
3. Ota T, et al. Nat. Genet. 36:40-45(2004).
4. Nielsen A.L, et al. J. Biol. Chem. 277:29983-29991(2002).
5. Singh R, et al. Genomics 82:185-193(2003).
6. Brenner M, et al. Nat. Genet. 27:117-120(2001).
7. Brockmann K, et al. Eur. Neurol. 50:100-105(2003).
8. Stumpf E, et al. Arch. Neurol. 60:1307-1312(2003).
9. Sawaishi Y, et al. Neurology 58:1541-1543(2002).
10. Aoki Y, et al. Neurosci. Lett. 312:71-74(2001).
PDF Data Sheet

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MSDS

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