biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact usFlyers

Technical Notes
View our tech notes
Follow us...
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive

Rabbit antibody to human IBA1: whole serum


Catalogue No. R-1820-50
Description Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. Ref:
Batch No. See product label.
Unit size 50 uL
Antigen C-terminal peptide of human IBA1 protein coupled to KLH.
Antibody Type Polyclonal
Other Names Allograft inflammatory factor 1; AIF-1; Ionized calcium-binding adapter molecule 1; Protein G1
Accession P55008 (AIF1_HUMAN)
Produced in Rabbit
Molecular Weight IBA1 is detected at ~15-17 kDa on western blot.
Purity Whole serum
Applications Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:2,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Reacts with human, rat, mouse.
Form Lyophilised.
Reconstitution Spin vial briefly before opening. Reconstitute in 50 uL sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Rabbit antibody to human IBA1: whole serum Left: Visualization of microglia (green) with anti-IBA antibody (1:1,000) by Immunohistochemistry. Image represents Z-stacked confocal image of rat cerebellar molecular layer at top and granular layer below. Microglia in their "surveilling" state demonstrate a small cell soma with fine, elongated processes spreading in three dimension. Red: MAP2-immunoreactivity in processes of Purkinje cells and the perikarya of granule cells, revealed with chicken anti-MAP2 antibody C-1382-50 (1:5,000). Blue: Nuclear DNA stained with DAPI. Right: Western blot analysis of tissue homogenates using rabbit anti-IBA1 antibody (1:1,000). Lane 1: Molecular weight standard; Lane 2: mouse brain; Lane 3: rat brain; Lane 4: mouse spleen; Lane 5: rat spleen. IBA1 appears at ~15-17 kDa. IBA1 is a relatively minor protein of brain and is much more abundant in spleen, as evidenced by different band intensities. The other bands seen in the CNS homogenates are of unknown origin, but do not appear to compromise the migroglia-specific staining seen with this antibody.

PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet


Click to download the MSDS - Click to download the MSDS

Offices in the USA and Australia
My Ice Bucket  
0 items
Email This Page
Tell someone you know about this product.
We Accept PayPal
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment

Official PayPal Seal