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Rabbit antibody to human Ki-67: whole serum

$297.00USD


Catalogue No. R-1806-100
Description Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). Ref: uniprot.org
Batch No. See product label.
Unit size 100 L
Antigen Recombinant human Ki-67 protein (amino acids 1,111-1,490) expressed in and purified from E. coli.
Other Names Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67
Accession P46013 (KI67_HUMAN)
Produced in Rabbit
Purity Whole serum
Applications Western blotting (1:2,000-1:10,000) and Immunocytochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Reacts with human only. Does not react with mouse or rat.
Form Lyophilised, without preservatives.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 L sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Rabbit antibody to human Ki-67: whole serum Left: Immunofluorescence analysis of HeLa cells costained with rabbit antibody to Ki-67 (1:5,000, red) and a mouse antibody to beta-tubulin. Ki-67 is predominantly localized in nucleoli of cells in interphase, while in mitotic cells Ki-67 is associated with condensed chromosomes. Ki-67 is not expressed in cells in quiescent G0 state. Blue: DAPI staining of nuclear DNA. Right: Western blots analysis of equal amounts of HeLa (Lane 2) and HEK293 (Lane 3) cell lysates using rabbit antibody to Ki-67 (1:10,000). Double bands above 250 kDa correspond to two major Ki-67 isoforms of molecular weight ~345 and 395 kDa. Smaller fragments of these isoforms are also detected on the blot.

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MSDS

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