biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact us
 


Technical Notes
 
View our tech notes
 
 
Follow us...
 
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive
 
 

Rabbit antibody to MAP1LC3A : whole serum

$297.00USD


Catalogue No. R-146-100
Description MAP1A and MAP1B are microtubule-associated protein which mediate the physical interactions between microtubules and components of the cytoskeleton (probably involved in autophagosome formation). MAP1A and MAP1B each consist of a heavy chain subunit and 3 different light chain subunits (LC1, LC2 and LC3). MAP1LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor form of MAP1LC3A is cleaved by APG4/ATG4B to form the cytosolic form LC3-1. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. MAP1LC3A is most abundant in heart, brain, liver, skeletal muscle and testis but is absent in thymus and peripheral leukocytes.
Batch No. See product label
Unit size 100 l
Antigen A synthetic peptide (RSFADRCKEVQQI) corresponding to the N-terminal of human MAP1LC3 A protein conjugated to Blue Carrier Protein has been used as the immunogen. The sequence is homologous with mouse and rat MAP1LC3 A.
Other Names Microtubule-associated proteins 1A/1B light chain 3A; MAP1A/MAP1B LC3 A; MAP1A/1B light chain 3 A; MAP1 light chain 3-like protein 1; Microtubule-associated protein 1 light chain 3 alpha; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; APG8a; MAP1LC3A
Accession MLP3A_HUMAN
MLP3A_MOUSE
MLP3A_RAT
Produced in Rabbit
Molecular Weight ~20 kDa uncleaved, 14-18kDa cleaved, SDS-PAGE
Purity Whole serum
Applications IHC, immunohistochemistry on 4% PFA fixed or 2% PLP fixation, 1:100-1000 primary antibody dilution. IC, Immunocytochemistry: acetone fixed specimens recommended. 1:100-1:1000 primary antibody dilution. Western Blot: R-146-100 requires samples to be denatured ONLY by boiling in SDS solution, not reduced. No signal is achieved using reduced samples. (Samples treated with DTT or Beta mercaptoethanol). 1:500 overnight 4 degrees is recommended for primary antibody dilution in western blots. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity IHC, WB and ELISA confirmed the specificity for MAP1LC3 A.
Cross-reactivity Human, rat. Other species not yet tested.
Form Lyophilised
Reconstitution Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for higher stability, and at 4C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
References 1. The MGC Project Team. Genome Res. 14:2121-2127(2004)
Images (click to zoom)
Rabbit antibody to MAP1LC3A : whole serum Confocal microscopy on immunofluorescently detected MAP1LC3 A  in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 A : whole serum (R-146-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for MAP1LC3 A appearing in red and MHC class II  appearing in green. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Rabbit antibody to MAP1LC3A : whole serum PC12 non-reduced samples (boiled in SDS only, no DTT or BME)
Rabbit antibody to MAP1LC3A : whole serum A. Confocal microscopy on immunofluorescently detected MHC class II in cytospin-isolated human white blood cells.
Rabbit antibody to MAP1LC3A : whole serum B. Confocal microscopy on immunofluorescently detected MAP1LC3 A in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 A : whole serum (R-146-100) at a dilution of 1: 200, incubated for 1 h at room temperature.
Rabbit antibody to MAP1LC3A : whole serum C is the merged picture of Figures A & B. Confocal microscopy on immunofluorescently detected MAP1LC3 A in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 A : whole serum (R-146-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for MAP1LC3 A appearing in red and MHC class II appearing in green. Here, the merged picture is presented.
Rabbit antibody to MAP1LC3A : whole serum Confocal microscopy on immunofluorescently detected MAP1LC3 A in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 A : whole serum (R-146-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for MAP1LC3 A appearing in red and MHC class II appearing in green. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Rabbit antibody to MAP1LC3A : whole serum Confocal microscopy on immunofluorescently detected MAP1LC3 A in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 A: whole serum (R-146-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Rabbit antibody to MAP1LC3A : whole serum Confocal microscopy on immunofluorescently detected MAP1LC3 A in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 A : whole serum (R-146-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for MAP1LC3 A appearing in red and MHC class II appearing in green. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Rabbit antibody to MAP1LC3A : whole serum Confocal microscopy on immunofluorescently detected MAP1LC3 A in cytospin-isolated human white blood cells using Rabbit antibody to MAP1LC3 A : whole serum (R-146-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for MAP1LC3 A appearing in red and MHC class II appearing in green. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Rabbit antibody to MAP1LC3A : whole serum Western blot on Peripheral Blood Mononuclear Cells (PMBC) lysate using Rabbit antibody to MAP1LC3 A : whole serum (R-146-100) at a dilution of 1: 100 (ECL).
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

Customers who bought this product also purchased
Rabbit antibody to Beclin-1: whole serum
Rabbit antibody to Beclin-1: whole serum

Sheep antibody to rh Basic FGF: affinity purified
Sheep antibody to Connexin-37 (266-281): whole serum
Sheep antibody to Connexin-37 (266-281): whole serum
Rabbit antibody to ATG5: IgG
Rabbit antibody to ATG5: IgG
Rabbit antibody to human capsaicin receptor (608-621): whole serum
Rabbit antibody to human capsaicin receptor (608-621): whole serum
Mouse monoclonal antibody to human p75NTR [MLR2]: IgG2a
Mouse monoclonal antibody to human p75NTR [MLR2]: IgG2a

Offices in the USA and Australia
My Ice Bucket  
 
0 items
 
 
Currencies
 
  
 
 
Email This Page
 
 
Tell someone you know about this product.
 
 
We Accept PayPal
 
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment


Official PayPal Seal