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Rabbit antibody to rh NT4: IgG

$297.00USD


Catalogue No. R-102-500
Description FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Batch No. See product label
Unit size 500 ug
Antigen Recombinant human NT4
Other Names Neurotrophin-5; NT-5; Neutrophic factor 5; Neurotrophin-4; NT-4; Neutrophic factor 4; NTF5; NTF4
Accession NT5_HUMAN
Produced in Rabbit
Purity Protein G purified IgG
Applications IHC, ELISA (1 site), Western Blot, ICC, inhibition of biological activity in vitro/in vivo. Recommended to be used at a concentration of 1-10 ug/mL for immunohistochemistry, ELISA, ICC and Western blot and inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 ug/mL (ED50). Note that the concentration of NT4 is generally low in most tissues nevertheless, neonatal testes of rat can be used as a good positive control. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot.
Cross-reactivity Known to react with NT4 from rat and human, mouse and monkey.
Blast it If you would like to see the shared identity between different species or other proteins follow the link in Accession field, select then the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
Form Lyophilised
Reconstitution Reconstitute in 500 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Expiry Date 12 months after purchase
References 1. Lodouichi (2000) J. Neuroscie. 20(6): 2155
2. Zhang et al. (1999) J Neurosci. Meths. 89 (1): 69
Images (click to zoom)
Rabbit antibody to rh NT4: IgG Immunohistochemical staining of Neurotrophin 4/5 (NT4/5) in rat cortex using rabbit polyclonal to recombinant human NT4, catalogue number R-102-500
Rabbit antibody to rh NT4: IgG Immunohistochemical staining of Neurotrophin 4/5 (NT4/5) in rat cortex using rabbit polyclonal to recombinant human NT4, catalogue number R-102-500
Rabbit antibody to rh NT4: IgG Immunofluorescence analysis of NT4 expression in human DU145 prostate cancer cells. Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X100) DU145 cells were incubated with NT4 rabbit antibody antibody R-102-500 (10 μg/mL, green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-rabbit-CF488A antibody (4 μg/mL, 1 hour incubation). Control cells were incubated with normal rabbit IgG (10 μg/mL) as primary antibody. Cell nuclei were stained with Hoechst dye (blue). Magnification: 100x.
PDF Data Sheet

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MSDS

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