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Rabbit antibody to Peroxiredoxin-6 (NSGPx): whole serum

$297.00USD


Catalogue No. R-169-100
Description FUNCTION: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. Lysosome. Also found in lung secretory organelles. MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin . MISCELLANEOUS: Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. SIMILARITY: Belongs to the ahpC/TSA family. Rehydrin subfamily.
Batch No. See product label
Unit size 100 uL
Antigen Rat recombinant Peroxiredoxin-6. The sequence is homologous in human and mouse peroxiredoxin-6.
Other Names Non-selenium glutathione peroxidase; antioxidant protein 2; 1-Cys peroxiredoxin; 1-Cys PRX; Acidic calcium-independent phospholipase A2; NSGPx; Thiol-specific antioxidant protein; Prdx6; Aipla2; Aop2; Tsa
Accession PRDX6_RAT
PRDX6_HUMAN
PRDX6_MOUSE
Produced in Rabbit
Purity Whole serum
Applications IHC, WB, ELISA. This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 1000 depending on tissue and detection method. For western blotting a dilution range of 1: 1000 to 1: 4000 is recommended. A dilution of 1: 1000 to 1: 4000 is recommended for ELISA. This antiserum stains the cytoplasm of epithelial cells in the rat and mouse lung and rat and human brain astrocytes. It stains human brain astrocytes in Parkinson's and Alzheimer's disease and the central core of some Lewy bodies in Parkinson's disease and dementia with Lewy bodies. Other tissues have not yet been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity This antibody has been shown to be specific for Peroxiredoxin-6 protein.
Cross-reactivity Rat, human and mouse, other species have not yet been tested.
Blast it If you would like to use this product in another speices other than those specified here, or to see the shared identity between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form Lyophilised
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Expiry Date 12 months after purchase
Specific References 1. Walsh B. et al. (2010) Overexpression of Prdx6 and resistance to peroxide-induced death in Hepa1-6 cells: Prdx suppression increases apoptosis. Redox Rep. 2009;14(6):275-84.
2. Gardiner F. et al. (2010) Induction of Prdx1 and Prdx6 in Liver Cells by Serum and TPA. Intl J. Biol. Vol. 2, No. 1
References 1. Kim T.-S, et al. J. Biol. Chem. 272:2542-2550(1997).
2. Choi H.-J, et al. Nat. Struct. Biol. 5:400-406(1998).
3. Rush J, et al. Nat. Biotechnol. 23:94-101(2005).
4. Wagner E, et al. Biochem. J. 366:777-785(2002).
5. Chen J.-W, et al. J. Biol. Chem. 275:28421-28427(2000).
6. Kang S.W, et al. J. Biol. Chem. 273:6303-6311(1998).
7. Gevaert K, et al. Nat. Biotechnol. 21:566-569(2003).
8. Gregory S.G, et al. ature 441:315-321(2006).
9. Nagase T, et al. DNA Res. 2:37-43(1995).
10. Frank S, et al. Oncogene 14:915-921(1997).
11. Power J, et al. Am J. Path. 161: 885-894 (2202).
12. Power J, et al. Experimental Lung Res. 25: 379-391(1999).
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MSDS

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