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Rabbit antibody to recombinant EGFP: whole serum

$297.00USD


Catalogue No. R-110-100
Description FUNCTION: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000. SUBUNIT: Monomer. TISSUE SPECIFICITY: Photocytes. PTM: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. BIOTECHNOLOGY: Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. SIMILARITY: Belongs to the GFP family.
Batch No. See product label
Unit size 100 uL
Antigen Recombinant EGFP
Other Names Recombinant Enhanced Green Fluorescence Protein
Accession EGFP protein
GFP_AEQVI
Produced in Rabbit
Purity Whole serum
Applications IHC, Western blot. Recommended to be used at a dilution of 1:1000-1: 5000 for both applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity This antibody is known to react with EGFP confirmed by IHC and WB.
Blast it If you would like to see the shared identity between different species or other proteins follow the link in Accession field, select then the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
Form Lyophilised
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage Store lyophilized antibody at 2-8C. After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
References 1. Chalfie, M., Tu, Y. et al (1994) Science 263, 802-805
2. Tsien, R. Y. (1998) Annu Rev Biochem 67, 509-544
3. Dardalhon, V. et al (1999) Hum Gene Ther 10, 5-14
4. Kain, S. R. (1999) DDT 4, 304-312
Images (click to zoom)
Rabbit antibody to recombinant EGFP: whole serum Western blot of EGFP protein and EGFP in PC12 cell lysate (20 μg/lane) using R-110-100. Rabbit antibody R-110-100 detects EGFP monomer at around 30 kDa and shows low levels of non-specific bands. SDS-PAGE: denatured and reduced; Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 μm); Blocking: 5% skim milk in TBST, 1 hour at RT; Primary antibody: overnight at 2-8°C at indicated dilutions in blocking buffer; Secondary antibody: anti-rabbit-HRP (1/6000) 1 hour at RT; Detection: Chemiluminiscence.

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MSDS

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