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Rabbit antibody to Saporin: whole serum

$297.00USD


Catalogue No. R-025-100
Description Saporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins. The expected molecular weight of the purified saporin is 29.5 kDa.
Batch No. See product label
Unit size 100 l
Antigen Saporin, whole molecule
Other Names Saponaria officinalis; Common soapwort
Accession RIP6_SAPOF
Produced in Rabbit
Purity Whole serum
Applications IHC, Immunofluorescence, ELISA, Western Blot. A dilution of 1:200 to 1: 2000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Confirmed to react with purified saporin.
Cross-reactivity No cross-reactivity with other molecules has been reported.
Form Lyophilised
Reconstitution Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 4C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Expiry Date 12 months after purchase
References 1. Stirpe, F. et al (1983) Biochem J 216, 617-625
2. Tazzari, P. L. et al (1988) Cancer Immunol Immunother. 26, 231-236
3. Strocchi, P. et al (1992) J Immunol Methods. 155, 57-63
4. Savino, C. et al (2000) FEBS Lett. 470, 239-243
5. Barbieri, L. et al (2000) J Biochem (Tokyo). 128, 883-889
6. Savino, C. et al (1998) Acta Crystallogr D Biol Crystallogr. 54, 636-638
7. Santanche, S. et al (1997) Biochem Biophys Res Commun. 234, 129-132
Images (click to zoom)
Rabbit antibody to Saporin: whole serum Western blot analysis using rabbit polyclonal anti-saporin antisera (R-025-100). HRP-labelled sheep anti-rabbit was used as the secondary antibody and the immunocomplex was then visualised by DAB. The expected molecular weight of the purified saporin is 29.5 kDa. SC: Spinal Cord homogenate (rat).

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