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Rabbit antibody to the Tyrosine Kinase Receptor A (TrkA): whole serum

$297.00USD


Catalogue No. R-152-100
Description TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Cell membrane; single-pass type I membrane protein. Endocytosed to the endosomes upon treatment of cells with NGF. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. Both isoforms have similar biological properties. TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells. Isoform TrkA-I is found in non-neuronal tissues. Mutations in TrkA have been associated with congenital insensitivity to pain, anhidrosis, self-mutalating behaviour, mental retardation and cancer.
Batch No. See product label
Unit size 100 uL
Antigen Extracellular domain of glycosylated human TrkA protein produced in mammalian cells was used as the immunogen.
Other Names Tropomyosin-related kinase receptor; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1 transforming tyrosine kinase protein; p140-TrkA; Trk-A; NTRK1; TRK;
Accession NTRK1_HUMAN
Produced in Rabbit
Purity Whole serum
Applications IHC (1:1000 dilution), Western Blotting (denaturing and non-reducing SDS-PAGE, 1:1000-1:2500 dilution), 1-site ELISA (1:5000 dilution). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Specificity was demonstrated by immunohistochemistry. When used for immunohistochemistry in rat dorsal root ganglia, staining is restricted to the known distribution of TrkA, that is in small, nociceptive neurons.
Cross-reactivity Reacts with human, rat and mouse TrkA. Other species not yet tested.
Form Lyophilised
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Specific References Kobayashi K et al. (2015) Nerve Growth Factor Signals as Possible Pathogenic Biomarkers for Perineural Invasion in Adenoid Cystic Carcinom. Otolaryngol Head Neck Surg. 2015 Aug;153(2):218-24. Species: Human. Application: IHC.

Uren RT et al. (2014) A novel role of suppressor of cytokine signaling-2 in the regulation of TrkA neurotrophin receptor biology. J Neurochem. 2014 May;129(4):614-27. Species: Mouse. Application: IF.
References 1. Zhang FX, et al. (2005) Brain Res 16; 1062 (1-2) pp. 92-100.
2. Gearhart DA, et al. (2006) J Neurosci Methods. 150(2) pp. 159-173.
Images (click to zoom)
Rabbit antibody to the Tyrosine Kinase Receptor A (TrkA): whole serum Immunohistochemical staining of Tyrosine Kinase Receptor A (TrkA) in rat spinal cord (free floating cryostat section) using rabbit antibody (R-152-100) at a dilution of 1 in 2000. Courtesy of Professor Xin Fu Zhou, The Flinders University of South Australia.
Rabbit antibody to the Tyrosine Kinase Receptor A (TrkA): whole serum Immunohistochemical staining of Tyrosine Kinase Receptor A (TrkA) in rat trigeminal nerve (free floating cryostat section) using rabbit antibody (R-152-100) at a dilution of 1 in 2000. Courtesy of Professor Xin Fu Zhou, The Flinders University of South Australia.
Rabbit antibody to the Tyrosine Kinase Receptor A (TrkA): whole serum Western blot of TrkA receptor in human neuroblastoma SH-SY5Y cell lysate (30 ug/lane). R-152-100 detects TrkA full-length protein at 130-140 kDa under non-reducing conditions (no DTT or beta-mercaptoethanol!). Higher molecular-weight bands may represent homodimeric forms of TrkA or heterodimeric TrkA-receptor complexes. The identity of lower molecular weight bands is unknown. SDS-PAGE: denatured and non-reducing; Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 um); Blocking: 5% skim milk in TBST, 1 hour at RT; Primary antibody: overnight at 4C (1/2500 dilution); Secondary antibody: anti-rabbit-HRP (1/6000) 2 hours at RT; Detection: Chemiluminiscence. Predicted MW of humanTrkA based on amino acid sequence: 84 kDa. The observed MW differs due to post-translational modification, mainly glycosylation.
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