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Rabbit antibody to Tyrosine Kinase Receptor B (TrkB; 70-90): affinity purified

$327.00USD


Catalogue No. R-1834-100
Description The protein named TrkB (also named Neurotrophic tyrosine kinase receptor type 2 (NTRK2), GP145-TrkB or Tropomyosin-related kinase B is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems and is important in the regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. TrkB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. TrkB is the primary receptor for BDNF (brain-derived neurotrophic factor. TrkB also binds NT4 and NT3 but less efficiently. Upon ligand-binding, the receptor undergoes homodimerization, autophosphorylation and activation. TrkB activation recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that each regulate distinct overlapping signaling cascades within cells. Through SHC1, FRS2, SH2B1, SH2B2, these activate the GRB2-Ras-MAPK cascade that regulates, for instance, neuronal differentiation including neurite outgrowth. These same effectors also control the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. TrkB, via activation of PLCG1 and the downstream protein kinase C-regulated pathways, also controls synaptic plasticity, and thus plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. One such consequence is that PLCG1 activation via TrkB is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. (Reference: www.uniprot.org)
Batch No. See product label
Unit size 100 ug
Antigen Synthetic peptide immunogen, EIFIANQKRLEIINEDDVEAY
Sequence EIFIANQKRLEIINEDDVEAY
Antigen Location Extracellular domain of TrkB (amino acids 70-90 of human TrkB)
Antibody Type Rabbit polyclonal
Other Names GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B
Accession NTRK2_HUMAN
Produced in Rabbit
Molecular Weight This antibody detects TrkB full-length (~140 kDa) and truncated TrkB-T1 isoforms (~90 kDa) as determined by Western Blotting.
Purity Affinity purified
Applications Western Blotting (0.1 - 0.5 ug/mL): Tested on RIPA lysates from human and rodent brain tissue.
Flow Cytometry (5-20 ug/mL): Tested on human cell lines.
Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity TrkB
Species Against Human.
Cross-reactivity Mouse and rat TrkB. Species cross-reactivity determined by Western Blotting.
Form Lyophilized from a solution containing 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 uL sterile distilled water to obtain an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened
Images (click to zoom)
Rabbit antibody to Tyrosine Kinase Receptor B (TrkB; 70-90): affinity purified Western blot analysis of TrkB expression in various human and rodent tissue homogenates (RIPA extracts). Two prominent bands are detected at ~140 kDa and ~90 kDa, corresponding to TrkB full-length (FL) and truncated TrkB (T1) isoforms. SDS-PAGE: denatured and reduced; Transfer: Towbin’s buffer (20% MeOH, no SDS); Membrane: PVDF (0.45 um); Blocking: 5% skim milk in TBST, 1 hour at RT; Primary antibody: overnight at 2-8C (0.5 ug/mL); Secondary antibody: anti-rabbit-HRP (1/10,000), 1 hours at RT; Detection: Chemiluminiscence.
Rabbit antibody to Tyrosine Kinase Receptor B (TrkB; 70-90): affinity purified Analysis of membrane TrkB expression in three human cell lines by Flow Cytometry. Staining was perfomed under native conditions. Blocking: 10% normal horse serum; Primary antibody: R-1834-100 (red), 10 ug/mL, 30 minutes on ice; Secondary antibody: donkey anti-rabbit PE (1:100), 30 minutes in dark on ice. Negative control: normal rabbit IgG (black). Data and results were generated using Orflo Moxiflow instrument and protocols.
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MSDS

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