biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact usFlyers
 


Technical Notes
 
View our tech notes
 
 
Follow us...
 
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive
 
 

Rabbit antibody to Tyrosine Kinase Receptor C (TrkC; 172-186): affinity purified

$327.00USD


Catalogue No. R-1835-100
Description Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation (Ref: uniprot.org).
Batch No. See product label
Unit size 100 ug
Antigen Synthetic peptide immunogen, QLWQEQGEAKLNSQN
Sequence QLWQEQGEAKLNSQN
Antigen Location Extracellular domain of TrkC (amino acids 172-186 of human TrkC)
Antibody Type Rabbit polyclonal
Other Names GP145-TrkC; Trk-C; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase
Accession NTRK3_HUMAN
Produced in Rabbit
Molecular Weight This antibody detects TrkC full-length (~140 kDa) and truncated TrkC.T1 isoforms (~100 kDa) as determined by Western Blotting.
Purity Affinity purified
Applications Western Blotting (0.1 - 0.5 ug/mL): Tested on RIPA lysates from human and rodent tissue and cell lysates.
Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity TrkC
Species Against Human
Cross-reactivity Mouse and rat TrkC. Species cross-reactivity determined by Western Blotting.
Form Lyophilized from a solution containing 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 uL sterile distilled water to obtain an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened
Images (click to zoom)
Rabbit antibody to Tyrosine Kinase Receptor C (TrkC; 172-186): affinity purified Western blot analysis of TrkC expression in various human and rodent tissue and cell extracts (RIPA). Two prominent bands are detected at ~140 kDa and ~100 kDa, corresponding to TrkC full-length (FL) and truncated TrkC (T1) isoforms. Additional, uncharacterized lower molecular-weight bands are observed. SDS-PAGE: denatured and reduced; Transfer: Towbin’s buffer (20% MeOH, no SDS); Membrane: PVDF (0.45 um); Blocking: 5% skim milk in TBST, 1 hour at RT; Primary antibody: overnight at 2-8C (1 ug/mL); Secondary antibody: anti-rabbit-HRP (1/10,000), 1 hours at RT; Detection: Chemiluminiscence.

PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

Offices in the USA and Australia
My Ice Bucket  
 
0 items
 
 
Currencies
 
  
 
 
Email This Page
 
 
Tell someone you know about this product.
 
 
We Accept PayPal
 
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment


Official PayPal Seal