||FUNCTION: Seems to promote the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
||See product label
||A synthetic peptide (YAEHKSHRGEY) as part of human (aa: 139-149), mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
||Neurotrophin-3; Neurotrophic factor; HDNF; Nerve growth factor 2; NGF-2; Ntf3; Ntf-3
||IHC, ELISA, WB, inhibition of biological activity. A dilution of 1:500 to 1:2000 is recommended for IHC, western blot. For inhibition of biological activity: 1:10-50 for in vitro, 5-10 uL/g body weight for in vivo. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot.
||This antiserum is known to react with rat, chicken and human NT3.
||If you would like to use this product in another species other than those specified here, or to see the shared ID between the immunogen used here in different species and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
||Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
||After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
||12 months after purchase
||1. Zouh et al. (1996) Mol. Neurobio. 13(3): 185-97
2. Rush et al. (1997) Clin. Exp. Pharmacol. 24: 549-55
3. Tafreshi et al. (1998) Neurosci. 83(2): 373-80