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Rabbit monoclonal antibody to Rho-associated protein kinase 1, ROCK1: Affinity purified

$297.00USD


Catalogue No. R-1791-100
Description Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.
Batch No. See product label
Unit size 100 g
Antigen A synthesized peptide derived from human ROCK1
Antibody Type Monoclonal
Other Names Renal carcinoma antigen NY-REN-35; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK
Accession Q13464 ROCK1_HUMAN;
Produced in Rabbit
Purity Affinity purified
Applications Western blotting 1:500-1:2000; IHC(P) 1:50-1:200, heat-mediated antigen retrieval required (20 min, citrate buffer pH 6); ICC/IF 1:50-1:200. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Species Against Human
Cross-reactivity Mouse and rat
Form Liquid. Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Antibody is shipped on blue ice. Upon receipt, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Rabbit monoclonal antibody to Rho-associated protein kinase 1, ROCK1: Affinity purified Left: Detection of ROCK1 expression in Ramos cell lysate by Western Blotting. Right: ROCK1 detection in paraffin-embedded human kidney tissue by IHC. Heat-mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 minutes.

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