||Autosomal recessive mutations in DJ-1 cause early-onset familial Parkinson's disease. DJ-1 is considered a redox-sensitive cytoplasmic protein found in brain as well as other cell types.
||See product label
||A synthetic peptide (ASKRALVILAKGAEE-C) corresponding to human DJ-1 [2-16] in the N-terminal domain conjugated via additional C-terminal Cys to Diphtheria toxoid.
||WB and IHC. Suggested dilution of 1:5,000 is recommended for WB. DJ-1 is a soluble protein of 189 amino acids and detected with 23 kDa mobility by western blotting. The suggested dilution for IHC is 1:100. Detected astrocyte cytoplasmic labelling in human brain formaldehyde-treated tissue. Biosensis recommends that the optimal working dilution should be determined by the end user.
||Confirmed by WB using soluble mouse and human brain extracts, reactivity for major product diminished by peptide absorption.
||Human and mouse DJ-1 are highly conserved, so cross-reactivity with other species is expected.
||Lyophilized from PBS, pH 7.4. Contains no preservative.
||Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
||Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
||12 months after purchase
||Sonia George, Su San Mok, Milawaty Nurjono, Scott Ayton, David I. Finkelstein, Colin L. Masters,Qiao-Xin Li & Janetta G. Culvenor (2010) alpha-Synuclein Transgenic Mice Reveal Compensatory Increases in Parkinson's Disease-Associated Proteins DJ-1and Parkin and Have Enhanced alpha-Synuclein and PINK1 Levels After Rotenone Treatment J Mol Neurosci 42:243_254