biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact us
 


Technical Notes
 
View our tech notes
 
 
Follow us...
 
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive
 
 

Rabbit polyclonal antibody to DJ-1/PARK7: IgG

$297.00USD


Catalogue No. R-1682-500
Description Autosomal recessive mutations in DJ-1 cause early-onset familial Parkinson's disease. DJ-1 is considered a redox-sensitive cytoplasmic protein found in brain as well as other cell types.
Batch No. See product label
Unit size 500 ug
Antigen A synthetic peptide (ASKRALVILAKGAEE-C) corresponding to human DJ-1 [2-16] in the N-terminal domain conjugated via additional C-terminal Cys to Diphtheria toxoid.
Antibody Type Polyclonal
Other Names PARK7
Produced in Rabbit
Applications WB and IHC. Suggested dilution of 1:5,000 is recommended for WB. DJ-1 is a soluble protein of 189 amino acids and detected with 23 kDa mobility by western blotting. The suggested dilution for IHC is 1:100. Detected astrocyte cytoplasmic labelling in human brain formaldehyde-treated tissue. Biosensis recommends that the optimal working dilution should be determined by the end user.
Specificity Confirmed by WB using soluble mouse and human brain extracts, reactivity for major product diminished by peptide absorption.
Species Against Human and mouse DJ-1 are highly conserved, so cross-reactivity with other species is expected.
Form Lyophilized from PBS, pH 7.4. Contains no preservative.
Reconstitution Reconstitute in 500 L of sterile water. Centrifuge to remove any insoluble material.
Storage Short term storage at 2-8C for one week. At -20C as an undiluted liquid for up to 12 months.
Expiry Date 12 months after purchase
General References Sonia George, Su San Mok, Milawaty Nurjono, Scott Ayton, David I. Finkelstein, Colin L. Masters,Qiao-Xin Li & Janetta G. Culvenor (2010) alpha-Synuclein Transgenic Mice Reveal Compensatory Increases in Parkinson's Disease-Associated Proteins DJ-1and Parkin and Have Enhanced alpha-Synuclein and PINK1 Levels After Rotenone Treatment J Mol Neurosci 42:243_254
Images (click to zoom)
Rabbit polyclonal antibody to DJ-1/PARK7: IgG Western blotting for DJ-1 in extracts of primary cultured neurons. Samples resolved on 10% Tris-tricine gels and transferred to nitrocellulose membrane for blotting.
Rabbit polyclonal antibody to DJ-1/PARK7: IgG Immunohistochemistry of formaldehyde-fixed human brain, paraffin sections treated with anti-DJ-1 at 1:1000.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

Customers who bought this product also purchased
Mouse monoclonal antibody to DJ-1 [PARK7]: IgG
Mouse monoclonal antibody to DJ-1 [PARK7]: IgG

Offices in the USA and Australia
My Ice Bucket  
 
0 items
 
 
Currencies
 
  
 
 
Email This Page
 
 
Tell someone you know about this product.
 
 
We Accept PayPal
 
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment


Official PayPal Seal