biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact us
 


Technical Notes
 
View our tech notes
 
 
Follow us...
 
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive
 
 

Rabbit polyclonal antibody to Apoptotic protease-activating factor 1 (2-28): Affinity purified

$297.00USD


Catalogue No. R-1342-100
Description
APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9, leading to the activation of caspase-3 and apoptosis (Ref: SWISSPROT).
Batch No. See product label
Unit size 100 g
Antigen A synthetic peptide (DAKARNCLLQHREALEKDIKTSYIMDH) corresponding to a region (2-28 aa) from the N-terminus of human Apoptotic protease-activating factor 1 (APAF1).
Sequence DAKARNCLLQHREALEKDIKTSYIMDH
Antibody Type Antiserum
Other Names APAF-1; APAF1; Apoptotic peptidase activating factor 1;
Accession O14727 APAF_HUMAN;
Produced in Rabbit
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 g/ml is recommended for WB. Human APAF1 has a predicted length of 1,248 residues and MW of 142 kDa. A concentration of 2.0 g/ml is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB (Human) and IHC (Rat) against the antigen.
Antibody Against Apoptotic protease-activating factor 1
Cross-reactivity Human (WB); rat (IHC-P); predicted to react with mouse due to sequence homology;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between proteins. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Blast URL Click here
Form Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Appearance Lyophilized white powder
Reconstitution Reconstitute in 100 l of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles
Expiry Date 12 months after purchase
Specific References Sezik M et al. (2016) "Maternal intravenous granulocyte-colony stimulating factor and intra-amniotic 1 high-dose endotoxin for the experimental caprine model of fetal brain injury" Turkish Journal of Medical Sciences DOI: 10.3906/sag-1505-28. Application: IHC. Species: Goat

Aydogan A et al. (2014) "Immunohistochemical expression of caspase-3, caspase-5, caspase-7 and apoptotic protease-activating factor-1 (APAF-1) in the liver and kidney of rats exposed to zoledronic acid (ZOL) and basic fibroblast growth factor (bFGF)." Vet Q. 2014;34(3):137-42. Application: IHC. Species: Rat
Images (click to zoom)
Rabbit polyclonal antibody to Apoptotic protease-activating factor 1 (2-28): Affinity purified
Western Blot using Rabbit polyclonal antibody to Apoptotic protease-activating factor 1:
Lane 1: MCF-7 Whole cell lysate
Lane 2: Hela Whole cell lysate
Lane 3: Jurkat Whole cell lysate
Lane 4: HT1080 Whole cell lysate
Lane 5: Colo320 Whole cell lysate

PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

Offices in the USA and Australia
My Ice Bucket  
 
0 items
 
 
Currencies
 
  
 
 
Email This Page
 
 
Tell someone you know about this product.
 
 
We Accept PayPal
 
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment


Official PayPal Seal