||BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycaemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
||See product label
||A peptide (HSDPARRGELSVCDSISEWV) corresponding to a sequence at the N-terminal of human BDNF (amino acids 129-148).
||Brain-derived neurotrophic factor; Abrineurin; proBDNF;
|| P23560 BDNF_HUMAN; P21237 BDNF_MOUSE; P23363 BDNF_RAT
||Affinity purified on antigen column
||Western Blotting (WB). A concentration of 0.1-0.5 ug/mL is recommended for WB. Human BDNF has a predicted length of 247 residues and MW of 28 kDa for BDNF precursor and 13 kDa for mature BDNF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||The specificity of this antibody has been confirmed by WB against the antigen.
||Human; mouse; rat;
||If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please chose carefully, as the sequence you chose may be a precursor rather than the mature protein for example.
||Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
||Maintain lyophilized material at -20C for up to 12 months after date of receipt. After reconstitution, aliquot and store at -20C for up to 6 months. Avoid freeze-thaw cycles.
||12 months after purchase, unopened.