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Rabbit polyclonal antibody to gamma-H2AX (phospho S140): Affinity purified

$297.00USD


Catalogue No. R-1555-100
Description
This product is no longer available. Please see below for our recommended alternative products.
Batch No. See product label
Unit size 100 µg
Antigen Synthetic peptide from a region surrounding phosphorylated serine 140 of human H2AX (134-143 aa).
Sequence KKATQASQEY
Other Names H2AX139ph; Histone H2A.x; H2a/x; H2AFX; H2AX; Histone H2AX; Histone H2AFX; Histone H2A/X; gamma H2AX;
Accession P16104 H2AX_HUMAN;
Produced in Rabbit
Applications Western Blotting at a suggested dilution of 1:1,000, Flow Cytometry (2ug/10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Species Against Human;
Cross-reactivity This antiserum detects phosphorylated H2AX only.
Blast URL Click here
Form Lyophilised
Reconstitution Reconstitute in 100 µl of sterile distilled water. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Rabbit polyclonal antibody to gamma-H2AX (phospho S140): Affinity purified A: Specific staining of gamma-H2AX expressed in human neuroblastoma SH-SY5Y cell line by Flow Cytometry using cat # R-1555-100. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 15% Horse serum, Primary antibody: Rabbit Polyclonal antibody to gamma-H2AX (2 μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Donkey anti-rabbit PE labeled secondary antibody (1:100 dilution) with incubation for 20 minutes in dark at room temperature. Negative Control: Normal Rabbit IgG (black dashed). Data and results were generated using Orflo MoxiflowTM instrument and protocols. B: Western Blot using Rabbit polyclonal antibody to gamma-H2AX (R-1555-100) in treated lymphocytes from human blood. The gamma-H2AX antibody at 1:1,000 in TBST and 3% BSA. Anti-HRP secondary at 1:2,000. Image taken with ImageQuant Las 4000.

PDF Data Sheet

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MSDS

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