p73 belongs to the p53 family of transcription factors and has roles in the apoptotic response to DNA damage. p73 is expressed at very low levels in normal tissues and is differentially expressed in a number of tumours. At least 35 variants, including isoform p73 alpha, arise as a result of alternate promoter usage, initiation sites and multiple splicing.
||See product label
||A synthetic peptide (QRVMEAVHFRVRHTIT) corresponding to a region (580-595) from human p73 alpha. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
||Tumor protein p73; p53-like transcription factor; p53-related protein; TP73; P73; p73;
||Affinity purified on antigen column
||Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 ug/mL is recommended for WB. Human p73 (isoform alpha) has a predicted length of 636 residues and MW of 70 kDa. A concentration of 1.0-2.0 ug/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Antigen retrieval is required.
||The specificity of this antibody has been confirmed by WB and IHC against the antigen.
||Human; mouse; rat;
||If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
||Liquid (0.5ml) in 50% glycerol, 0.9mg NaCl and 0.2mg Na2HPO4
||The liquid formulation should be diluted in PBS (pH 7.4)
||After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles
||12 months after purchase