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Rabbit polyclonal antibody to human APPL1 (455-709): Whole serum

$297.00USD


Catalogue No. R-1331-100
Description APPL1 (adaptor protein containing PH domain, PTB domain and leucine zipper motif 1) mediates adiponectin signaling in various types of cells. APPL1 is also thought to have a role in insulin signaling.
Batch No. See product label
Unit size 100 l
Antigen The C-terminus of Human APPL1 protein (amino acids: 455-709) conjugated to GST.
Other Names DCC-interacting protein 13-alpha; Dip13-alpha; Adapter protein containing PH domain; PTB domain and leucine zipper motif 1; APPL1; APPL; DIP13A; KIAA1428;
Accession Q9UKG1 DP13A_HUMAN;
Produced in Rabbit
Purity Whole serum
Applications Western Blotting (WB) and Immunoprecipitation (IP). A dilution of 1:1,000 is recommended for WB. A concentration of 3 l/reaction is recommended for IP. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Cross-reactivity This antiserum is known to recognise both mouse and human APPL1.
Blast URL Click here
Form Lyophilised
Appearance White powder
Reconstitution Reconstitute in 100 l of sterile distilled water. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
References 1. Mao X. et al (2006) APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function Nat Cell Biol. 2006 May;8(5):516-23.
2. Wang C. et al (2009) Yin-Yang regulation of adiponectin signaling by APPL isoforms in muscle cells J Biol Chem. 2009 Nov 13;284(46):31608-15.
3. Zhou L. et al (2009) Adiponectin activates AMP-activated protein kinase in muscle cells via APPL1/LKB1-dependent and phospholipase C/Ca2+/Ca2+/calmodulin-dependent protein kinase kinase-dependent pathways J Biol Chem. 2009 Aug 14;284(33):22426-35
Images (click to zoom)
Rabbit polyclonal antibody to human APPL1 (455-709): Whole serum Western Blot showing APPL1 expression in mammalian cells. Lysates from HEK293 (HEK), mouse hepatocytes (HEP), differentiated C2C12 myotubes (C2C12), HeLa, INS1 and L6 cells were separated by SDS–PAGE and examined by western blot using Rabbit polyclonal antibody to human APPL1, catalog number R-1331-100. Image courtesy of Mao X. et al (2006) Nat Cell Biol.
Rabbit polyclonal antibody to human APPL1 (455-709): Whole serum Western blot analysis with Rabbit polyclonal antibody to human APPL1, catalog number R-1331-100. Tissue homogenates of mouse brain (B), liver (Li), muscle (M), fat (F), heart (H), kidney (K), spleen (S) and pancreas (P) were used and 30g of protein was loaded in each lane. Image courtesy of Wang C. et al (2009) J Biol Chem.
PDF Data Sheet

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MSDS

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