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Rabbit polyclonal antibody to human Rho-associated protein kinase 2, ROCK2 (652-923): Affinity purified

$297.00USD


Catalogue No. R-1789-100
Description Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca2+ sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
Batch No. See product label
Unit size 100 g
Antigen E.coli-derived human ROCK2 recombinant protein (amino acids E652-D923).
Antibody Type Polyclonal
Other Names Rho kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK-II; p164 ROCK-2
Accession O75116 ROCK2_HUMAN;
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 g/mL is recommended for WB. Human Rho-associated protein kinase 2 has a predicted length of 1388 amino acids and MW of 161 kDa. A concentration of 0.5-1.0 g/mL is recommended to detect the protein in paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Human ROCK2.
Cross-reactivity Mouse and rat.
Form Lyophilised with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3
Reconstitution Reconstitute in 100 L of sterile distilled water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage Store lyophilized antibody at 2-8C. After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Rabbit polyclonal antibody to human Rho-associated protein kinase 2, ROCK2 (652-923): Affinity purified A: Detection of ROCK2 in tissue and cell lysates (40-50 ug protein per lane) by Western Blotting. Lane 1: Rat brain; Lane 2: Rat Liver; Lane 3: HELA; Lane 4: CEM; Lane 5: A549; Lane 6: MCF-7. B-D: ROCK2 detection by IHC(P) in mouse cardiac muscle tissue (B), rat brain tissue (C) and human mammary cancer tissue (D).

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