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Rabbit polyclonal antibody to human C-X-C Chemokine receptor 4 (18-33): Affinity purified


Catalogue No. R-1143-100
Description The C-X-C Chemokine receptor 4 (CXCR4) is a multi-pass membrane protein that belongs to the G-protein coupled receptor 1 family. CXCR4 is a specific receptor for stromal cell-derived factor-1 (SDF-1) and is widely expressed in tissues including peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla. At least 2 isoforms are produced by alternative splicing.
Batch No. See product label
Unit size 100 g
Antigen A synthetic peptide (SGDYDSMKEPCFREEN) corresponding to a region (18-33) from human C-X-C Chemokine receptor 4. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Other Names C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; Stromal cell-derived factor 1 receptor; SDF-1 receptor; Fusin; Leukocyte-derived seven transmembrane domain receptor; LESTR; LCR1; FB22; NPYRL; HM89; CD184; CXCR4;
Accession P61073 CXCR4_HUMAN;
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 g/ml is recommended for WB. Human CXCR4 (isoform 1) has a predicted length of 352 residues and MW of 40 kDa. A concentration of 0.5-1.0 g/ml is recommended to detect the protein in human formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval (Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins) is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human (WB, IHC); rat (WB); predicted to react with mouse due to sequence homology;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please chose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Reconstitution Reconstitute in 100 l of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C for up to six months. Avoid freeze-thaw cycles
Expiry Date 12 months after purchase (lyophilized)
PDF Data Sheet

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