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Rabbit polyclonal antibody to human Cullin-4B (877-895): Affinity purified

$297.00USD


Catalogue No. R-1141-100
Description Cullin-4B is a core component of E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of specific proteins in the cell.
Batch No. See product label
Unit size 100 g
Antigen A synthetic peptide (DRDYMERDKENPNQYNYIA) corresponding to a region (877-895) from the C-terminus of human Cullin-4B. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Other Names CUL-4B; CUL4B; KIAA0695;
Accession Q13620 CUL4B_HUMAN;
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Western Blotting (WB) and Immunohistochemistry (IHC). A concentration of 1.0 g/ml is recommended for WB. Human Cullin-4B (isoform 1) has a predicted length of 895 residues and MW of 102 kDa. A concentration of 2.0 g/ml is recommended for IHC in Zebra fish. Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Zebrafish; Human; Rat;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practice is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Reconstitution Reconstitute in 100 l of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Rabbit polyclonal antibody to human Cullin-4B (877-895): Affinity purified Western blot analysis of rabbit polyclonal antibody to human Cullin-4B at 1μg/ml:

Lane 1: Zebra fish tissue lysate
Lane 2: MM453 whole cell lysate
Lane 3: Rat liver tissue lysate
Lane 4: Rat lung tissue lysate
Lane 5: MCF7 whole cell lysate
Lane 6: MM231 whole cell lyate

Secondary HRP conjugated goat anti-rabbit IgG antibody (working dilution: 1:3000-10000).

PDF Data Sheet

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MSDS

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