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Rabbit polyclonal antibody to human Leu-Enkephalin (230 - 234)

$297.00USD


Catalogue No. R-1498-50
Description Leu-Enkephalin is cleaved from the precursor Proenkephalin-A. Leu-Enkephalin is an endogenous opioid peptide that interacts with opioid receptors and produces analgesic effects.
Batch No. See product label
Unit size 50 ug
Antigen Synthetic human Leu-Enkephalin peptide (230 - 234) conjugated to BSA.
Sequence YGGFL
Antigen Location 230-234
Other Names Proenkephalin-A; PENK;
Accession P01210 PENK_HUMAN;
Produced in Rabbit
Applications A concentration of 5-10 ug/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A concentration of 5-15 ug/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Species Against Human
Antibody Against Leu-Enkephalin
Cross-reactivity Human; mouse; rat. Leu-Enkephalin is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Met-enkephalin 0.93%; with beta-lipotropin 0.01%; with beta-endorphin 0.01%
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between proteins. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Blast URL Click here
Form Lyophilised with BSA
Appearance White powder
Reconstitution Reconstitute in 0.05 mL of PBS (pH 7.4). Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2-8C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability for up to six months and at 2-8C with an appropriate antibacterial agent for up to one week. Avoid freeze-thaw cycles
Expiry Date 12 months after purchase unopened.
Images (click to zoom)
Rabbit polyclonal antibody to human Leu-Enkephalin (230 - 234) Immunohistochemical staining in rat spinal cord dorsal horn. 4% paraformaldehyde fixed rat brain crystostat sections (10 µm) were incubated overnight at 4°C with Rabbit polyclonal antibody to human Leu-Enkephalin (10 µg/ml) followed by incubation with donkey anti-rabbit Rhodamine Red conjugated secondary antibody (1:200).

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MSDS

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