||Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) and several isoforms of GAD67 and GAD65 have been discovered. Clustal alignments show 64.1% amino acid identity between GAD65 and GAD67 across the entire protein with higher identity being toward the C-terminal ends of the proteins. Expression of the two proteins and of the specific isoforms (for instance isoform 3 of GAD67 is not expressed in brain) also varies. This antibody reacts with GAD67 and has not yet been tested directly on recombinant GAD65 for cross reactivity. Blots of whole brain extracts appear as a single band at 67 kDa. The sequence used as an immunogen does have some identity with GAD65 however it is slight and broken up by multiple gaps.
||See product label
||E.coli-derived human GAD67 recombinant protein (14-122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67.
NAGADPN TTNLRPTTYD TWCGVAHGCT RKLGLKICGF LQRTNSLEEK SRLVSAFKER QSSKNLLSCE NSDRDARFRR TETDFSNLFA
RDLLPAKNGE EQTVQFLLEV VD
||GAD; GAD-67; GAD1; Glutamate decarboxylase 1;
||Affinity purified on antigen column
||Western Blotting (WB). A concentration of 0.1-0.5 ug/mL is recommended for WB. The detection limit for GAD67 is approximately 0.25ng/lane under reducing conditions. Human GAD67 has a predicted length of 594 residues and MW of 67 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.Not yet tested in other applications.
||The specificity of this antibody has been confirmed by WB against HeLa cell, human colo320 cell and whole rat brain extracts. Antibody detects 67kDa single band under normal exposure, reduced SDS-PAGE conditions.
||Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
||Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
||At -20C for one year (Lyophilized). After reconstitution, the antibody can be stored at 2-8C for one month or alternatively, aliquot the vial into smaller volumes and store at -20C for a higher stability. Avoid repeated freezing and thawing.
||12 months after purchase.