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Rabbit polyclonal antibody to human Growth-associated protein 43 (216-238): Affinity purified

$327.00USD


Catalogue No. R-1055-100
Description Growth-associated protein 43 (GAP-43) is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is also a major component of neuronal growth cones. GAP-43 binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Phosphorylation of GAP-43 by a protein Kinase C is correlated with certain forms of synaptic plasticity. GAP-43 is used to specifically label injured neurons and to score neuronal regeneration.
Batch No. See product label
Unit size 100 ug
Antigen A synthetic peptide corresponding to a region (216-238) from human Growth-associated protein 43 (GAP-43). To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Other Names Neuromodulin; GAP43; PP46; p57; B-50; F1; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50;
Accession P17677 NEUM_HUMAN
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5ug/mL is recommended for WB. Human GAP-43 has a predicted length of 238 residues and MW of 25 kDa. A concentration of 0.5-1ug/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is recommended.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human; rat; predicted to react with mouse;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 added as preservative
Reconstitution Reconstitute in 100 uL of sterile distilled water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2-8C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles
Expiry Date 12 months after purchase
PDF Data Sheet

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MSDS

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