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Rabbit polyclonal antibody to human Heat Shock 70 kDa Protein 1 (469-487): Affinity purified

$327.00USD


Catalogue No. R-1149-100
Description Heat Shock 70 kDa protein 1 belongs to the Heat Shock protein 70 family. This chaperone protein is expressed in response to heat shock and stabilizes existing proteins against aggregation as well as mediating the folding of newly translated proteins.
Batch No. See product label
Unit size 100 ug
Antigen A synthetic peptide (RGVPQIEVTFDIDANGILN) corresponding to a region (469-487) from human Heat Shock 70 kDa Protein 1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Other Names Heat shock 70 kDa protein 1; HSP70.1; HSP70-1; HSP70-2; HSPA1A; HSPA1; HSPA1B; FLJ54328;
Accession P08107 HSP71_HUMAN;
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 ug/mL is recommended for WB. Human Heat Shock 70 kDa Protein 1 has a predicted length of 641 residues and MW of 70 kDa. A concentration of 1.0-2.0 ug/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Reconstitution Reconstitute in 100 uL of sterile distilled water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2-8C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
PDF Data Sheet

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MSDS

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