biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact usFlyers
 


Technical Notes
 
View our tech notes
 
 
Follow us...
 
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive
 
 

Rabbit polyclonal antibody to human Heat Shock Protein beta-1 (180-194): Affinity purified

$327.00USD


Catalogue No. R-1085-100
Description
Heat shock protein beta-1 (HSPB1) belongs to the small heat shock protein (HSP20) family. HSPB1 is expressed in response to heat shock and is also activated by estrogen in hormone responsive cells. HSPB1 is expressed in most cells with highest expression in heart and in tissues composed of striated and smooth muscle.
Batch No. See product label
Unit size 100 ug
Antigen A synthetic peptide (TIPVTFESRAQLGGP) corresponding to a region (180-194) from human Heat shock protein beta-1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Other Names Heat shock protein beta-1; HspB1; Heat shock 27; HSP 27; Stress-responsive protein 27; SRP27; Estrogen-regulated 24 kDa protein; 28 kDa heat shock protein; HSPB1; HSP27; HSP28;
Accession P04792 HSPB1_HUMAN;
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 ug/mL is recommended for WB. Human HSPB1 has a predicted length of 205 residues and MW of 23 kDa. A concentration of 0.5-1.0 ug/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human; expected to react with rat and mouse due to sequence homology
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Liquid (0.5ml). 50% glycerol, 0.9mg NaCl and 0.2mg Na2HPO4
Reconstitution The liquid formulation should be diluted in PBS (pH 7.4)
Storage After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles
Expiry Date 12 months after purchase
Images (click to zoom)
Rabbit polyclonal antibody to human Heat Shock Protein beta-1 (180-194): Affinity purified Immunohistochemical staining of paraffin-embedded human mammary cancer sections using Heat shock protein beta-1 antibody (R-1085-100) at a concentration of 1-2μg/ml.  Secondary detection with Biotinylated goat anti-rabbit IgG (10μg/ml).

PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

Offices in the USA and Australia
My Ice Bucket  
 
0 items
 
 
Currencies
 
  
 
 
Email This Page
 
 
Tell someone you know about this product.
 
 
We Accept PayPal
 
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment


Official PayPal Seal