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Rabbit polyclonal antibody to human Insulin receptor subunit alpha (59-75): Affinity purified

$297.00USD


Catalogue No. R-1124-100
Description
The Insulin receptor binds insulin and has a tyrosine-protein kinase activity. The receptor is a tetramer of 2 alpha and 2 beta subunits linked by disulfide bonds. The alpha subunit (135 kDa) is extracellular and contributes to the formation of the ligand-binding domain whereas the beta subunit (95 kDa) contains the kinase domain and a transmembrane domain. At least two isoforms of the Insulin receptor are produced by alternative splicing.
Batch No. See product label
Unit size 100 g
Antigen A synthetic peptide (HLQILLMFKTRPEDFRD) corresponding to a region (59-75) from human Insulin receptor (isoform Long). To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Other Names IR; EC 2.7.10.1; CD220; INSR;
Accession P06213 INSR_HUMAN;
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 g/ml is recommended for WB. Human Insulin receptor (isoform Long) has a predicted length of 1382 amino acids and MW of 156 kDa. A concentration of 0.5-1.0 g/ml is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Boiling the paraffin sections in 10mM citrate buffer, pH 6.0 for 20 minutes is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human; dog; rat; predicted to react with mouse due to sequence homology;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Reconstitution Reconstitute in 100 l of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability for up to 6 months. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase unopened
Images (click to zoom)
Rabbit polyclonal antibody to human Insulin receptor subunit alpha (59-75): Affinity purified Immunohistochemical staining of a paraffin-embedded rat cardiac muscle tissue section with rabbit polyclonal antibody to human Insulin receptor subunit alpha antibody (1-2 ug/ml). Secondary biotinylated goat anti-rabbit IgG antibody (10 ug/ml).

PDF Data Sheet

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MSDS

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