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Rabbit polyclonal antibody to human Matrix Metalloproteinase 2 (MMP2, 475-490): Affinity Purified

$297.00USD


Catalogue No. R-1381-100
Description Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. MMP2 isoform 2 mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways. Catalytic activity of MMP2 causes cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln. (Ref: uniprot.org).
Batch No. See product label.
Unit size 100 ug
Antigen Human C-terminal peptide (amino acids 475-490).
Antibody Type Polyclonal.
Isotype IgG
Other Names 72 kDa gelatinase; Gelatinase A; MMP-2; TBE-1
Accession P08253 MMP2_HUMAN;
Produced in Rabbit
Purity Affinity purified.
Applications Western Blotting (WB): 1:5,000 - 1:10,000. MMP2 appears as two bands at apparent molecular weights of 66 and 72 kDa.

Immunohistochemistry (IHC): 1:500-1:1,000.

Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Human.
Cross-reactivity Expected to react with horse, cow, pig, chicken, rat and mouse MMP2.
Form Lyophilised from PBS pH 7.4, containing 3% trehalose, without preservatives.
Reconstitution Reconstitute with 100 uL sterile-filtered, ultrapure water, to achieve an antibody concentration of 1 mg/mL. Centrifuge briefly to remove any insoluble material.
Storage Store lyophilised, unopened vial at 2-8C or lower. After reconstitution, prepare aliquots and store at -20C to -80C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase (unopened vial).
Images (click to zoom)
Rabbit polyclonal antibody to human Matrix Metalloproteinase 2 (MMP2, 475-490): Affinity Purified A: Western blot analysis of MMP2 expression in crude homogenate from injured rat peripheral nerve (right lane). Right lane: pro- and active form of recombinant human MMP2 protein.
B: Immunohistochemistry. Cross-section of injured rat nerve showing MMP2 expression by Schwann cells and other reactive cells.

PDF Data Sheet

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MSDS

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