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Rabbit polyclonal antibody to human S-phase kinase-associated protein 2 (2-20): Affinity purified

$297.00USD


Catalogue No. R-1102-100
Description S-phase kinase-associated protein 2 (SKP2) is a subunit in the SKP1-CUL1-F-box protein E3 ubiquitin protein ligase complex. This complex mediates the ubiquitination and the subsequent degradation of proteins involved in cell cycle progression, signal transduction and transcription.
Batch No. See product label
Unit size 100 g
Antigen A synthetic peptide (HRKHLQEIPDLSSNVATSF) corresponding to a region (2-20) from the N-terminus of human S-phase kinase-associated protein 2. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Other Names F-box protein Skp2; Cyclin A/CDK2-associated protein p45; p45skp2; F-box/LRR-repeat protein 1; SKP2; FBXL1;
Accession Q13309 SKP2_HUMAN;
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 g/ml is recommended for WB. Human SKP2 (Isoform SKP2_alpha) has a predicted length of 424 residues and MW of 48 kDa. A concentration of 1.0-2.0 g/ml is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human; mouse; rat;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Reconstitution Reconstitute in 100 l of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles
Expiry Date 12 months after purchase
Images (click to zoom)
Rabbit polyclonal antibody to human S-phase kinase-associated protein 2 (2-20): Affinity purified Immunohistochemical staining of paraffin-embedded human mammary cancer sections using rabbit polyclonal antibody to human S-phase kinase-associated protein 2 (1-2 μg/ml). Secondary biotinylated goat anti-rabbit IgG antibody (10 μg/ml).

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MSDS

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