||The Receptor for Advanced Glycation Endproducts (RAGE) is also known as the Advanced glycosylation end product-specific receptor (AGER). RAGE is expressed in endothelial cells and interacts with many molecules including advanced glycosylation end products (AGE) and amyloid beta peptide. At least 2 RAGE isoforms are produced by alternative splicing. Isoform 1 is a single-pass type I membrane protein whereas isoform 2 is secreted.
||See product label
||A synthetic peptide corresponding to a region (178-196 aa) from human RAGE.
||Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0µg/ml is recommended for WB. Human RAGE (isoform 1) has a predicted length of 404 residues and MW of 43 kDa. A concentration of 1.0 µg/ml is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||The specificity of this antibody has been confirmed by WB and IHC against the antigen.
||Human (WB, IHC), Rat (IHC). Predicted to react with mouse due to sequence homology
||Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
||Reconstitute in 100 µl of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
||At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
||12 months after purchase