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Rabbit polyclonal antibody to human Sonic Hedgehog protein (34-50): Affinity purified

$327.00USD


Catalogue No. R-1082-100
Description Sonic Hedgehog protein (SHH) is essential for a variety of patterning events during embryo development. SHH is expressed in fetal intestine, liver, lung, and kidney. The SHH precursor protein is autocatalytically cleaved into two products. The C-terminal portion is involved in the proteolysis of the precursor and cholesterol transferase activity. The N-terminal portion contains signalling activity.
Batch No. See product label
Unit size 100 ug
Antigen A synthetic peptide (RHPKKLTPLAYKQFIPN) corresponding to a region (34-50) from human Sonic Hedgehog protein (SHH). To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Other Names SHH; HHG-1;
Accession Q15465 SHH_HUMAN;
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 ug/mL is recommended for WB. Human Sonic Hedgehog protein (precursor) has a predicted length of 462 amino acids and MW of 50 kDa. A concentration of 1.0-2.0 ug/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human; mouse; rat; rabbit;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Reconstitution Reconstitute in 100 uL of sterile distilled water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2-8C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles
Expiry Date 12 months after purchase
Images (click to zoom)
Rabbit polyclonal antibody to human Sonic Hedgehog protein (34-50): Affinity purified Immunohistochemical detection of SHH in paraffin-embedded rat brain tissue sections using Rabbit polyclonal antibody to Sonic Hedgehog protein (R-1082-100) at 1.0-2.0 µg/ml. Secondary antibody was biotinylated goat anti-rabbit at a concentration of 10.0 µg/ml.
Rabbit polyclonal antibody to human Sonic Hedgehog protein (34-50): Affinity purified Western blot of SHH antibody R-1082-100 at 1-2μg/ml in rat brain tissue lysate. Secondary antibody HRP conjugated goat anti-rabbit IgG at 1:3000-10000.
PDF Data Sheet

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MSDS

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