| Catalogue No. |
R-1045-100 |
| Description |
The uptake of iron in cells occurs via the receptor-mediated endocytosis of the Transferrin receptor (TfR) bound to diferric transferrin into endosomes. Endosomal acidification leads to the release of the iron. The transferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and a loss of affinity of apotransferrin for its receptor. Soluble Transferrin receptor (sTfR) is a serum circulating truncated form of the membrane receptor protein. |
| Batch No. |
See product label |
| Unit size |
100 ug |
| Antigen |
A synthetic peptide corresponding to a region (745-760) from human Transferrin Receptor. To enhance the immunological response, this peptide was coupled to carrier protein BSA. |
| Other Names |
CD71;Transferrin receptor protein 1; TfR1; TfR; TR; Trfr; T9; p90; |
| Accession |
P02786 TFR1_HUMAN; |
| Produced in |
Rabbit |
| Purity |
Affinity purified on antigen column |
| Applications |
Western Blotting (WB) and Immunohistochemistry. A concentration of 1.0 ug/mL is recommended for WB. Human Transferrin Receptor (membrane form) has a predicted length of 760 residues and MW of 85 kDa. A concentration of 1.0 ug/mL is recommended for IHC. Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by WB and IHC against the antigen. |
| Cross-reactivity |
Human; rat; |
| Blast it |
If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please chose carefully, as the sequence you chose may be a precursor rather than the mature protein for example. |
| Form |
Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 |
| Reconstitution |
Reconstitute in 100 uL of sterile distilled water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material. |
| Storage |
At least 12 months after purchase at 2-8C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles |
| Expiry Date |
12 months after purchase |
