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Rabbit polyclonal antibody to Myristoylated alanine rich C kinase substrate (MARCKS): Whole serum

$297.00USD


Catalogue No. R-1380-50
Description Myristoylated alanine-rich C-kinase substrate (MARCKS) is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. Ref: SWISSPROT.
Batch No. See product label
Unit size 50 uL
Antigen Recombinant full length human MARCKS expressed and purified from E. coli
Antibody Type Antiserum
Other Names Myristoylated alanine-rich C-kinase substrate; MARCKS; Protein kinase C substrate 80 kDa protein;Marcks; Macs;
Accession P30009 MARCS_RAT;
Produced in Rabbit
Applications Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB.
Species Against Human
Antibody Against Myristoylated alanine rich C kinase substrate (MARCKS)
Cross-reactivity Does not react with rodent protein.
Blast URL Click here
Form Lyophilised
Reconstitution Reconstitute in 50 uL sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Rabbit polyclonal antibody to Myristoylated alanine rich C kinase substrate (MARCKS): Whole serum Image of mixed neuron/glial cultures stained with Rabbit polyclonal antibody to Myristoylated alanine rich C kinase substrate (MARCKS) R-1380-50 (red) and Chicken polyclonal antibody to MAP2 C-1382-50. Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.
Rabbit polyclonal antibody to Myristoylated alanine rich C kinase substrate (MARCKS): Whole serum Left: Analysis of MARCKS expression (red) in HeLa cells by Immunocytochemistry. MARCKS protein was detected with rabbit antibody to MARCKS at 1:1,000 dilution. Cells were co-stained with a mouse anti-beta tubulin antibody (green). Blue: DAPI nuclear stain. The MARCKS antibody recognizes protein localized in the plasma membrane and cytoplasm. Right: Western blot analysis of MARCKS expression (green) in various cell line lysates using rabbit anti-MARCKS antibody (1:1,000). [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, [7] C6. The blot was probed simultaneously with mouse antibody to GAPDH (M-1376-250, red, 1:5,000). The strong band at 80 kDa corresponds to MARCKS protein, detected only in the proteins of human origin. Slight reactivity is observed in the monkey cells (lane 5), but no reactivity is seen on rodent cells (lanes 6 and 7). The GAPDH antibody used as a loading control reveals a single band at 37 kDa in all preparations.
PDF Data Sheet

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MSDS

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