biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact us
 


Technical Notes
 
View our tech notes
 
 
Follow us...
 
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive
 
 

Rabbit polyclonal antibody to NeuN/Fox3 (1-99): Whole serum

$297.00USD


Catalogue No. R-3770-100
Description Fox3 is one of a family of mammalian homologues of Fox-1. The Fox proteins are about 46kDa in size, and each includes a central highly conserved RRM type RNA recognition motif. Much interest has focused on Fox3 as a result of the recent finding that this protein corresponds to NeuN, a neuronal nuclear antigen. NeuN/Fox-3 has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei and cytoplasm. Our antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli. We did not use full length Fox3 as immunogen since the three mammalian Fox homologues, namely Fox1, Fox2 and Fox3, include virtually identical RRM motifs. The N-terminal region of the three molecules are much more variable in the three molecules so antibodies specific for each of the three molecules can therefore be generated.
Batch No. See product label
Unit size 100 l
Antigen Antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli.
Antigen Location 1-99
Antigen Length 100 amino acids
Antibody Type Antiserum
Other Names Feminizing locus on X; Fox-1; Fox3; NeuN;
Accession A6NFN3 FOX1C_HUMAN;
Produced in Rabbit
Applications Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500 - 1:1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IC. Two alternate transcripts can be seen at 46 kDa and 48 kDa in WB. This antibody has been used successfully for immunohistochemistry on paraformaldehyde fixed rat brain cortex (adult) tissue. It does not bind to the nuclei of perikarya of non-neuronal cells making it a good marker to identify neurons.
Antibody Against NeuN/Fox3
Cross-reactivity Rat. Predicted to react with other mammals due to sequence homology.
Blast URL Click here
Form Lyophilised
Appearance White powder
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Specific References Hao XZ et al (2016) Combining systemic and stereotactic MEMRI to detect the correlation between gliosis and neuronal connective pathway at the chronic stage after stroke. J Neuroinflammation. 13(1):156 Species: Rat, Application IH.

Zhou XF et al (2015) Transcription factors COUP-TFI and COUP-TFII are required for the production of granule cells in the mouse olfactory bulb.Development. 142(9):1593-605 Species: Mouse, Application IH.

Saha M, et al (2013) Spinal Mitogen-Activated Protein Kinase Phosphatase-3 (MKP-3) is Necessary for the Normal Resolution of Mechanical Allodynia in a Mouse Model of Acute Postoperative Pain. J. Neurosci. 33(43):17182-7

Liu X et al (2013) Suppressive Effect of Phenol Red on the Epileptiform Burst Activity via Activation of Estrogen Receptors in Primary Hippocampal Culture. PLoS ONE 8(4):e60189

Landry R.P. et al (2012) Spinal Cannabinoid Receptor Type 2 Agonist Reduces Mechanical Allodynia and Induces Mitogen-Activated Protein Kinase Phosphatases in a Rat Model of Neuropathic Pain. J Pain. 2012 Aug 14

Ndong C. et al (2012) Mitogen activated protein kinase phosphatase-1 prevents the development of tactile sensitivity in a rodent model of neuropathic pain. Mol Pain. 2012 Apr 27;8(1):34.
General References Kim K.K. et al (2009) Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors. J Biol Chem. 2009 Nov 6;284(45):31052-61.
Images (click to zoom)
Rabbit polyclonal antibody to NeuN/Fox3 (1-99): Whole serum Paraformaldehyde fixed frozen section of adult rat cortex stained with Rabbit polyclonal antibody to NeuN/Fox3 (R-3770-100) in red and counterstained for DNA in blue. Note that this Fox3 antibody stains neuronal nuclei and distal perikarya and does not bind to the nuclei of perikarya of non-neuronal cells and so can be used to identify neurons.
Rabbit polyclonal antibody to NeuN/Fox3 (1-99): Whole serum Fox3/NeuN immunoreactivity was detected in rat spinal cord with the R-3770-100 primary antibody (1:10,000). Photo courtesy of Dr. Xidao Wang, UCSF, San Francisco, CA.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

Customers who bought this product also purchased
Mouse monoclonal antibody to Glial Fibrillary Acidic Protein  (GFAP) [5C10]: IgG
Mouse monoclonal antibody to Glial Fibrillary Acidic Protein (GFAP) [5C10]: IgG

Offices in the USA and Australia
My Ice Bucket  
 
0 items
 
 
Currencies
 
  
 
 
Email This Page
 
 
Tell someone you know about this product.
 
 
We Accept PayPal
 
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment


Official PayPal Seal