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Rabbit polyclonal antibody to Neuron specific enolase: Whole serum

$297.00USD


Catalogue No. R-1396-50
Description Enolase is a metalloenzyme that catayzes the reaction between 2-phospho-D-glycerate and phosphoenolpyruvate during glycolysis. Mammalian enolase is composed of 3 subunits; alpha, beta and gamma (Neuron-specific enolase). These subunits can form homodimers or heterodimers. The alpha/gamma heterodimer and the gamma/gamma homodimer are found primarily in neurons.
Batch No. See product label
Unit size 50 uL
Antigen Recombinant human Neuron Specific Enolase (NSE) expressed in and purified from E.coli
Antibody Type Polyclonal
Other Names Gamma-enolase; EC 4.2.1.11; 2-phospho-D-glycerate hydrolyase; Neural enolase; Neuron-specific enolase; NSE; Enolase 2; ENO2;
Accession P09104 ENOG_HUMAN;
Produced in Rabbit
Purity Antiserum
Applications Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Specifically recognizes ~47 kDa NSE protein in WB.
Species Against Human
Antibody Against Neuron specific enolase
Cross-reactivity Rat, mouse
Blast URL Click here
Form Lyophilised
Reconstitution Reconstitute in 50 uL sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Rabbit polyclonal antibody to Neuron specific enolase: Whole serum Image shows the human embryonic kidney cells line 293 (Hek293). The red channel shows staining with Rabbit polyclonal antibody to Neuron specific enolase R-1396-50, which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker with Mouse monoclonal antibody to ubiquitin C-terminal hydrolase 1 (UCHL1), M-1407-100. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.
Rabbit polyclonal antibody to Neuron specific enolase: Whole serum Left: Immunofluorescent analysis of mixed cortical neuron-glial cell culture from E20 rat by Immunocytochemistry. Cells were stained with rabbit antibody to NSE (red, 1:500), and co-stained with chicken antibody to GFAP (C-1373-50, green, 1:5,000). Blue: Hoechst nuclear dye. The NSE antibody labels protein expressed in neuronal cells, while the GFAP antibody stains intermediate filaments in astrocytes. Right: Western blot analysis of tissue and cell lysates using rabbit antibody to NSE (green, 1:5,000). [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6. A single band at about 47 kDa corresponds to the NSE protein, seen only in extracts containing neurons or neuronal lineage cells.
PDF Data Sheet

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MSDS

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