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Rabbit polyclonal antibody to Nicastrin, central region: IgG

$297.00USD


Catalogue No. R-1685-500
Description Nicastrin, a type 1 membrane glycoprotein, is an essential component of the gamma secretase complex which is critical for the cleavage of the amyloid precursor protein and other membrane proteins. Nicastrin is widely expressed in different tissue types. This antibody reacts with immature forms of Nicastrin. Detection of higher mol. wt. mature forms is likely to be blocked by glycosylation in this region of the protein.
Batch No. See vial label
Unit size 500 ug
Antigen A synthetic peptide (C-QGETFDYIGSSRMVYD) corresponding to human Nicastrin [331-346] in the central region conjugated via additional N-terminal Cys to Diphtheria toxoid.
Antibody Type Polyclonal
Produced in Rabbit
Applications WB and IP. Suggested concentration of 3-10 ug/mL is recommended for WB. Human or mouse brain samples commonly prepared with reducing (50mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min. Unprocessed full length human Nicastrin is 709 amino acids, however this protein contains an N-terminal signal peptide which is considered to undergo removal/cleavage during processing and transit to the cell plasma membrane, in addition the protein undergoes glycosylation to produce a glycoprotein of about 145 kDa apparent MW by SDS PAGE. Biosensis recommends that the optimal working dilution should be determined by the end user.
Specificity Confirmed by WB using peptide absorption.
Species Against Human, mouse, rat and guinea pig. Nicastrin is highly conserved, so cross-reactivity with other species is expected.
Form Lyophilized from PBS, pH 7.4. Contains no preservative.
Reconstitution Reconstitute in 500 L of sterile water. Centrifuge to remove any insoluble material.
Storage Short term storage at 2-8C for one week. At -20C as an undiluted liquid for up to 12 months.
Expiry Date 12 months after purchase
References 1. Culvenor, J.G., Ilaya, N.T., Ryan, M.T., Canterford, L., Hoke, D., Williamson, N.A., McLean, C.A., Masters, C.L., and 1. Evin, G. (2004) Characterization of Presenilin complex from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with Presenilin mutations. Eur. J. Biochem. 271, 375-385.
2. Ilaya, N.T., Evin, G., Masters, C.L., and Culvenor, J.G. (2004) Nicastrin expression in mouse peripheral tissues is not co-ordinated with Presenilin and is high in muscle. J. Neurochem. 91, 230-237.
Images (click to zoom)
Rabbit polyclonal antibody to Nicastrin, central region: IgG Western Immunoblotting with nicastrin central region antibody of mouse Nicastrin protein in PS1-deficient cell line and adult mouse brain (right panel) in comparison with C-terminal Nicastrin antibody (Catalog number R-1684-500). Membrane proteins were prepared and loaded as 20 µg protein per lane.

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