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Rabbit polyclonal antibody to Peroxiredoxin-4 (65-83): Whole serum

$297.00USD


Catalogue No. R-1593-100
Description Peroxiredoxin-4 has a probable role in redox regulation of the cell.
Unit size 100 uL
Antigen A synthetic peptide (SRVSVADHSLHLSKAKISK) corresponding to a region (65-83 aa) from human Peroxiredoxin-4 conjugated to diptheria toxin.
Antibody Type Polyclonal
Other Names Peroxiredoxin-4; Antioxidant enzyme AOE372; AOE37-2; Peroxiredoxin IV; Prx-IV; Thioredoxin peroxidase AO372; Thioredoxin-dependent peroxide reductase A0372;
Accession Q13162 PRDX4_HUMAN;
Produced in Rabbit
Applications IHC, WB, ELISA. This antibody works in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for light microscopy are 1:500 to 1:1,000 and IF of 1:50 to 1:100. For WB a dilution range of 1: 1,000 to 1: 4,000 is recommended. A dilution of 1: 1,000 to 1: 4,000 is recommended for ELISA. This antibody stains non-ciliated bronchiolar cells in rat lung and what appears to be sebaceous glands in rat skin. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Cross-reactivity Human; rat;
Blast URL Click here
Form Lyophilized
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 8C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Specific References Tam KC et al. (2018) Evidence for the interaction of peroxiredoxin-4 with the store-operated calcium channel activator STIM1 in liver cells. Cell Calcium. 2018 May 15;74:14-28.
Images (click to zoom)
Rabbit polyclonal antibody to Peroxiredoxin-4 (65-83): Whole serum Rabbit polyclonal antibody to Peroxiredoxin-4 (R-1593-100) at 1/100 dilution in sebaceous glands in rat skin. Secondary antibody Jackson biotinylated Donkey anti-rabbit (1/2000) followed by Vector ABC kit and visualised with DAB substrate.

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MSDS

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