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Rabbit polyclonal antibody to Peroxisome proliferator-activated receptor gamma (76-93): Affinity purified

$297.00USD


Catalogue No. R-1361-100
Description PPARG is a nuclear hormone receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription (Ref: SWISSPROT).
Batch No. See product label
Unit size 100 g
Antigen A synthetic peptide (PHYEDIPFTRTDPVVADY) corresponding to a region (76-93 aa) from human Peroxisome proliferator-activated receptor gamma (PPARG).
Sequence PHYEDIPFTRTDPVVADY
Antibody Type Antiserum
Other Names PPAR-gamma; Nuclear receptor subfamily 1 group C member 3; PPARG; NR1C3;
Accession P37231 PPARG_HUMAN;
Produced in Rabbit
Applications Western Blotting (WB). A concentration of 1.0 g/ml is recommended for WB. Human PPARG (isoform 2) has a predicted length of 505 amino acids and MW of 58 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB against the antigen.
Antibody Against Peroxisome proliferator-activated receptor gamma
Cross-reactivity Human;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between proteins. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Blast URL Click here
Form Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Appearance Lyophilized white powder
Reconstitution Reconstitute in 100 l of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Rabbit polyclonal antibody to Peroxisome proliferator-activated receptor gamma (76-93): Affinity purified
Western Blot using Rabbit polyclonal antibody to Peroxisome proliferator-activated receptor gamma: Lane 1: MM453 Whole cell lysate
Lane 2: MM231 Whole cell lysate
Lane 3: HeLa Whole cell lysate
Lane 4: Jurkat Whole cell lysate
Lane 5: HT1080 Whole cell lysate

PDF Data Sheet

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MSDS

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