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Rabbit polyclonal antibody to Protein S100-B (58-64): Affinity purified

$297.00USD


Catalogue No. R-1345-100
Description
This product is no longer available. Please see below for our recommended alternative products.
Replaced by R-1734-100
Batch No. See product label
Unit size 100 g
Antigen A synthetic peptide corresponding to a region (58-64 aa) from human Protein S100 beta (S100b).
Antibody Type Antiserum
Other Names S100B; Calcium binding protein B1; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B;
Accession P04271 S100B_HUMAN;
Produced in Rabbit
Applications Western Blotting (WB) and Immunohistochemistry (IHC). A concentration of 1.0 g/ml is recommended for WB. Human S100b protein (precursor) has a predicted length of 92 amino acids and MW of 11 kDa. A concentration of 1.0 g/ml is recommended for IHC (paraffin). Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human (WB); rat (WB, IHC); predicted to react with mouse due to sequence homology;
Blast URL Click here
Form Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Appearance Lyophilized white powder
Reconstitution Reconstitute in 100 l of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles
Expiry Date 12 months after purchase
References Yasuda Y. et al. Relationship between S100beta and GFAP expression in astrocytes during infarction and glial scar formation after mild transient ischemia. Brain Res. 2004 Sep 17;1021(1):20-31.
Images (click to zoom)
Rabbit polyclonal antibody to Protein S100-B (58-64): Affinity purified
Western Blot using Rabbit polyclonal antibody to Protein S100-B:
Lane 1: Rat brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: MCF-7 Whole cell lysate
Lane 4: Hela Whole cell lysate
Lane 5: Smmc Whole cell lysate
Lane 6: Jurkat Whole cell lysate
Lane 7: Colo320 Whole cell lysate

PDF Data Sheet

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MSDS

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