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Rabbit polyclonal to CD44 (aa728-742) / Phagocytic glycoprotein 1 / PGP-1: affinity purified IgG

$327.00USD


Catalogue No. R-1721-100
Description The protein identified as CD44 is also known as the receptor for hyaluronic acid (HA). The protein mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, CD44 may play an important role in invadopodia formation, and it also is involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction of CD44 causes numerous pathogenic phenotypes. CD44 has great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Biosensis' anti-CD44 rabbit polyclonal antibody is a new antibody made against a synthetic peptide epitope near the very C-terminus, cytoplasmic domain of human CD44. The antibody has been shown to react with multiple species including human, mouse and rat, in both western blot and immunohistochemistry applications including formalin fixed, paraffin embedded tissues. See applications detail for specific use instructions.
Batch No. see vial label
Unit size 100 ug
Antigen A synthetic peptide corresponding to a sequence at the C-terminus of human CD44 cytoplasmic domain aa 728-742. The immunogen differs by only single amino acid between human, mouse and rat proteins.
Sequence DETRNLQNVDMKIGV
Antigen Location cytoplasmic, C-terminus
Antigen Length 15 aa
Antibody Type polyclonal
Isotype IgG
Other Names CDw44,Epican,Extracellular matrix receptor III,ECMR-III,GP90 lymphocyte homing/adhesion receptor,HUTCH-I,Heparan sulfate proteoglycan,Hermes antigen,Hyaluronate receptor,Phagocytic glycoprotein 1,PGP-1,Phagocytic glycoprotein I,PGP-I,CD_antigen: CD44
Accession P16070
Produced in Rabbit
Molecular Weight Variable depending upon splicing, 80- to 250-kDa
Purity Affinity purified over peptide
Applications WB: Western blot: reduced RIPA samples or membrane preparations (0.1-.5 ug/mL) dependent upon antigen load and antibody incubation times and detection substrate used.

IH: frozen or acetone fixed tissue: 0.5-1.0 ug/mL, permeabilization is necessary as epitope is internal
IH(P): 1-2 ug/mL with HEIR treatment required.
Form lyophilized.
Appearance Each vial contains 100ug purified antibody, and 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution 0.2 mL of distilled water will yield a concentration of 500 ug/mL.
Storage At -20C for one year. After reconstitution, at 2-8C for one month. It can also be divided into aliquots and stored frozen at -80C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months after purchase.
PDF Data Sheet

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MSDS

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