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Sheep antibody to c-FOS (2-17): affinity purified


Catalogue No. S-045-50
Description FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain
Batch No. See product label
Unit size 50 ug
Antigen A synthetic peptide (MFSGFNADYEASSSRC; aa 2-17) conjugated to diphtheria toxoid has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from cFos protein human, rat, mouse, hamster and cat.
Other Names Proto-oncogene protein cFOS; c-FOS
Accession FOS_HUMAN
Produced in Sheep
Purity Affinity purified
Applications IH on PFA fixed, frozen tissues. Not yet tested on formalin fixed, paraffin embedded tissue but expected to react. Affinity purified antibody is extremely powerful. A concentration of 1 -5 ug/mL is recommended most uses with short (1-8 hour) incubations. If using enhanced brightfield or amplified detection methods dilutions and long primary antibody incubations dilutions will need to be increased substantially to inhibit background staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Not recommended for western blotting applications. Mouse monoclonal antibody M-1752-100 or rabbit polyclonal antibody R-1751-50 are excellent alternatives for western blotting.
Specificity This antiserum shows a high level of specificity for cFOS confirmed by immunohostochemistry.
Cross-reactivity This antiserum is known to react with rat, rabbit and hamster cFOS.
Blast it If you would like to use this product in another species other than those specified here, or to see the shared identity between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form Lyophilised
Reconstitution Reconstitute in 50 uL of sterile water. Centrifuge to remove any insoluble material.
Storage Store lyophilized product at 2-8C. After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
References 1. Vanstraaten et al (1983) Proc. Natl. Acad. Sci. 80: 3183
2. Minson J et al. (1994) Disinhibition of the rostral ventral medulla increases blood pressure and Fos expression in bulbospinal neurons. Brain Res. 1994 May 16;646(1):44-52.
Images (click to zoom)
Sheep antibody to c-FOS (2-17): affinity purified c-Fos was induced in the hypothalamic arcuate nucleus of an adult male Wistar rat with i.p. injection of N-methyl-D-aspartate. The brain was fixed by transcardiac perfusion of the rat with formaldehyde (4%). cFos immunoreactivity was detected on floated cryo-sections of the hypothalamic arcuate nucleus with the S-045-50 primary antibody (1:5000) using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

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