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Sheep antibody to rh Basic FGF: affinity purified

$297.00USD


Catalogue No. S-076-100
Description Fibroblast growth factors (FGFs) bind heparin and exhibit widespread mitogenic and neurotrophic activities in a variety of different cells including mesenchymal, neuroectodermal and endothelial cells. There are differences in the tissue distribution and concentration of these 2 growth factors. aFGF (FGF-1) and bFGF (FGF-2) are present in relatively high levels in CNS. aFGF is expressed by a subset of neuronal populations, while bFGF is expressed by astrocytes, both lack signal peptides. Human bFGF is a 17.2 kDa protein containing 155 amino acid residues. FGF-2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2. MISCELLANEOUS: This protein binds heparin more strongly than does aFGF. SIMILARITY: Belongs to the heparin-binding growth factors family.
Batch No. See product label
Unit size 100 ug
Antigen Recombinant human basic FGF
Other Names Heparin-binding growth factor 2; HBGF-2; Basic fibroblast growth factor; bFGF; Prostatropin; FGF2; FGFB
Accession FGF2_HUMAN
Produced in Sheep
Purity Affinity purified
Applications IHC. A concentration of 1 ug/mL is recommended for immunohistochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity A high level of specificity for bFGF was shown by immunohistochemistry for this antiserum.
Cross-reactivity This antibody is known to react with human, mouse and rat basic FGF.
Blast it If you would like to see the shared identity between different species or other proteins follow the link in Accession field, select then the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
Form Lyophilised
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
References 1. Abraham, et al. (1986) Science. 233(4763):545-8
2. Kurokawa, et al. (1987) FEBS Lett. 213(1):189-94
PDF Data Sheet

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MSDS

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