||FUNCTION: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low molecular weight diffuse from one cell to a neighboring cell. Connexin-37 may also mediate hyperexcitability followin nerve injury. SUBUNIT: A connexon is composed of a hexamer of connexins. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. TISSUE SPECIFICITY: Highly expressed in lung. SIMILARITY: Belongs to the connexin family. Alpha-type (group II) subfamily.
||See product label
||A synthetic peptide (aa: 266-281) as part of rat Connexin-37 protein conjugated to diphtheria toxoid has been used as the immunogen.
||Gap junction alpha-4 protein; Cx37; Gja4; Cxn-37
|| Q03190 CXA4_RAT
||Immunohistochemistry: Antibody detects Cx37 in rat tissues and arterial endothelial cells. This antibody was used at 1:100 - 1:250 in the original publication (see Rummery, NM et al 2002 for more details), but Biosensis recommends optimal dilutions/concentrations should be determined by the end user. In the original work the specificity of the antibody was shown by incubation either without primary antibody or with primary antibody that had previously been pre-incubated for 1 hour at room temperature with 10-fold excess by weight of the peptide against which the antibody was raised.
This antibody is not recommended for Western Blotting.
||This antiserum recognizes Connexin-37 in rat, other species not yet tested
||Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
||Store lyophilized antibody at 2-8C. After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
||12 months after purchase
Rummery NM, Hickey H, McGurk G, Hill CE. (2002) “Connexin37 is the major connexin expressed in the media of caudal artery.” Arterioscler Thromb Vasc Biol. 22(9):1427-32. PMID: 12231561
This antibody is referred to as Cx37/266 in Rummery, NM et al 2002.