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Oligomeric Amyloid-beta ELISA Kit (2 plates)


Catalogue No. BEK-2215-2P
  Find a 1 plate size of this product here. Enquire about bulk discounts for ten or more plates, at
Description The oligomeric form of Amyloid Beta peptide (Aβ, 1-42) has been closely linked to Alzheimer's Disease. Several ELISAs targeting Aβ have been developed; however, these ELISAs are known to cross-react with Amyloid Beta precursor protein (APP) and are poorly characterized against monomeric and oligomeric forms of the peptide. The Biosensis MOAB-2 antibody, developed by LaDu and co-workers (Youmans K. et al., 2012), has been shown to specifically detect Aβ, but not the precursor molecule APP. When utilized in ELISAs, the oligomeric form of Aβ peptide (o-Aβ) can be assayed independently of the other forms of the molecule when assayed with the MOAB-2 monoclonal antibody.

The Biosensis oligomeric Aβ ELISA kit is a sandwich ELISA that allows the preferential quantification of oligomeric Aβ peptides. This kit is exclusive to Biosensis and consists of a pre-coated mouse monoclonal anti-Aβ capture antibody (MOAB-2), a biotinylated MOAB-2 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of o-Aβ present in samples and protein standards. The purpose of this kit is the in vitro qualitative measurement of oligomeric Aβ peptide levels in brain extracts and CSF samples from both transgenic mice and humans relative to a known o-Aβ standard. The inclusion of a highly validated oligomeric standard results in a unique, ready-to-use ELISA kit.

This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Related products Oligomeric Human beta-Amyloid Abeta1-42 Peptide, Stabilized (PE-1750-1000)
Batch No. Refer to the product label.
Antigen The standard in this ELISA is preformed oligomeric amyloid beta, manufactured by Biosensis from synthetic, HFIP-treated human beta-amyloid peptide, amino acids 1-42. The stabilized oligomeric beta amyloid 1-42 control complex is also constructed from the same synthetic peptide standard material. No animal systems were used for their manufacture.
Other Names Beta-APP42; Beta-APP40; Beta-amyloid protein 42; Beta-amyloid protein 40; ABPP; APPI; Amyloid beta A4 protein; MOAB2; MOAB-2; Alzheimer's antibody; AB40; AB42; abeta.
Specificity Human. MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to Aβ residues 1-4 and is highly specific just to amyloid beta peptide.

The Biosensis o-Aβ Elisa detects Aβ oligomers as validated and described by Youmans KL et al (2012) and Rat by Combes M et al (2015).
Cross-reactivity Rat.
Storage Store at 2-8C
Expiry Date 12 months from purchase.
Specific References El-Sayed NA et al. (2019) "Design, synthesis, in vitro and in vivo evaluation of novel pyrrolizine-based compounds with potential activity as cholinesterase inhibitors and anti-Alzheimer's agents." Bioorg Chem. [Epub ahead of print] Application: ELISA, Species: Human. In-vitro screening of drug candidates.

Oh Joo Kweon, Young Chul Youn, Yong Kwan Lim, Mi-Kyung Lee, Hye Ryoun Kim (2019) "Clinical utility of serum hepcidin and iron profile measurements in Alzheimer's disease." J Neurol Sci. [In press] Application: ELISA, Species: Human serum.

Pacheco-Quinto J, Clausen D, Perez-Gonzalez R, Peng H, Meszaros A, Eckman CB, Levy E, Eckman EA (2018) "Intracellular metalloprotease activity controls intraneuronal Aβ aggregation and limits secretion of Aβ via exosomes." FASEB J. [Epub ahead of print] Application: ELISA, Species: Human cell line, mouse brain and organotypic brain slice cultures.

Oh SB, Kim MS, Park S, Son H, Kim SY, Kim MS, Jo DG, Tak E, Lee JY (2018) "Clusterin contributes to early stage of Alzheimer's disease pathogenesis." Brain Pathol. [Epub ahead of print] Application: ELISA, Species: Transgenic mouse brain homogenates.

S Liu, S Park, G Allington, F Prelli, Y Sun, M Marta-Ariza, H Scholtzova, G Biswas, B Brown, PB Verghese, PD Mehta, Y-U Kwon and T Wisniewski (2017) "Targeting Apolipoprotein E/Amyloid β Binding by Peptoid CPO_Aβ17-21 P Ameliorates Alzheimer's Disease Related Pathology and Cognitive Decline." Sci Rep. 7(1):8009 Application: ELISA, Species: Transgenic mouse brain homogenates.

M Cacciottolo, X Wang, I Driscoll, N Woodward, A Saffari, J Reyes, M L Serre, W Vizuete, C Sioutas, T E Morgan, M Gatz, H C Chui, S A Shumaker, S M Resnick, M A Espeland, C E Finch and J C Chen (2017) "Particulate air pollutants, APOE alleles and their contributions to cognitive impairment in older women and to amyloidogenesis in experimental models." Transl Psychiatry. Jan 31;7(1):e1022. Application: ELISA, Species: Extracts of E3FAD and E4FAD transgenic mouse brains.

Riya Thomas, Paulina Zuchowska, Alan W. J. Morris, Felecia M. Marottoli, Sangeeta Sunny, Ryan Deaton, Peter H. Gann, Leon M. Tai (2016) "Epidermal growth factor prevents APOE4 and amyloid-beta-induced cognitive and cerebrovascular deficits in female mice." Acta Neuropathol Commun. 4(1):111 Application: ELISA, Species: Tris-extracts of EFAD transgenic mouse brains.

Nor Faeizah Ibrahim, Daijiro Yanagisawa, Lina Wati Durani, Hamizah Shahirah Hamezah, Hanafi Ahmad Damanhuri, Wan Zurinah Wan Ngah, Mayumi Tsuji, Yuji Kiuchi, Kenjiro Ono, Ikuo Tooyama (2016) "Tocotrienol-Rich Fraction Modulates Amyloid Pathology and Improves Cognitive Function in AβPP/PS1 Mice." J Alzheimers Dis. [Epub ahead of print]. Application: ELISA, Species: Tris-extracts of mouse brain homogenates.

Jia Luo, Sue H. Lee, Lawren VandeVrede, Zhihui Qin, Manel Ben Aissa, John Larson, Andrew F. Teich, Ottavio Arancio, Yohan D'Souza, Ahmed Elharram, Kevin Koster, Leon M. Tai, Mary Jo LaDu, Brian M. Bennett and Gregory R. J. Thatcher (2016) "A multifunctional therapeutic approach to disease modification in multiple familial mouse models and a novel sporadic model of Alzheimer's disease." Molecular Neurodegeneration 2016 11:35. Application: ELISA, Species: Tris-extracts of EFAD transgenic mouse brains.

Weiguo Peng, Thiyagarajan M. Achariyar, Baoman Li, Yonghong Liao, Humberto Mestre, Emi Hitomi, Sean Regan, Tristan Kasper, Sisi Peng, Fengfei Ding, Helene Benveniste, Maiken Nedergaard, Rashid Dean (2016) "Suppression of glymphatic fluid transport in a mouse model of Alzheimer's disease." Neurobiology of Disease. Vol. 93, Pages 215-225 Application: ELISA, Species: TBSX-extracts of mouse cerebral cortex.

Mafalda Cacciottolo, Amy Christensen, Alexandra Moser, Jiahui Liu, Christian J. Pike, Conor Smith, Mary Jo LaDu, Patrick M. Sullivan, Todd E. Morgan, Egor Dolzhenko, Andreas Charidimou, Lars-Olof Wahlund, Maria Kristofferson Wiberg, Sara Shams, Gloria Chia-Yi Chiang (2016) "The APOE4 allele shows opposite sex bias in microbleeds and Alzheimer's disease of humans and mice." Neurobiology of Aging. Volume 37, January 2016, Pages 4757 Application: ELISA, Species: Tris-extracts of E3FAD and E4FAD transgenic mouse brains.

Combes M, Poindron P, Callizot N.(2015) "Glutamate protects neuromuscular junctions from deleterious effects of β-amyloid peptide and conversely: An in vitro study in a nerve-muscle coculture." J Neurosci. Res. 93(4):633-43 Application: ELISA, Species: Native Rat neurites & human muscle cell co-culture supernatants.

Seo, Dong Han, et al. (2015) "Plasma-enabled sustainable elemental lifecycles: honeycomb-derived graphenes for next-generation biosensors and supercapacitors." Green Chem. 17:2164-2171. Application: ELISA, Species: synthetic constructs.

Tai, LM (2014) "Amyloid-β Pathology and APOE Genotype Modulate Retinoid X Receptor Agonist Activity in vivo." J Biol Chem. 289(44):30538-55 Application: ELISA, Species: EFAD-Tg mice.

Liu Y, Liu X, Hao W, Decker Y, Schomburg R, Fulop L, Pasparakis M, Menger MD, Fassbender K. (2014) "IKKbeta Deficiency in Myeloid Cells Ameliorates Alzheimer's Disease-Related Symptoms and Pathology." (2014) J Neurosci. Sep 24;34(39):12982-99 Application: ELISA, Species: Transgenic Mouse brain lysates, supernatants.
General References Youmans KL et al. (2011) J Neurosci Methods 196(1): 51-9
Youmans KL et al. (2012) Mol Neurodegener. 16;7:8
Tai ML et al. (2013) J Biol Chem. 288(8): 5914-26
Good pathology summary:
Viola KL, Klein WL. (2015) Amyloid β oligomers in Alzheimer's disease pathogenesis, treatment, and diagnosis. Acta Neuropathol. 2015 Feb;129(2):183-206
Kit components The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Range 0.031 - 2 ng/mL
Kit protocol Click to download the kit protocolBEK-2215_as_at_Oct2018.pdf
MSDS Click to download the Material Safety Data SheetMSDS_Rapid_ELISA_as_at_Aug2017.pdf
Images (click to zoom)
Oligomeric Amyloid-beta ELISA Kit (2 plates) This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.

PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

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