Rabbit antibody to APG10: whole serum

$190.00USD

Catalogue No.:	R-145-100
Batch No.:	See product label
Unit size:	100 µl
Antigen:	A peptide corresponding to the C-terminal of human APG10 protein has been used as the immunogen.
Other Names:	Autophagy-related protein 10, APG10-like, GENE NAME: ATG10
Accession:	APG10_HUMAN
Description:	APG10 plays a role in autophagy. It is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: APG12-APG5 conjugations and modificatioon of a soluble form of MAPLC3 1A, a homolog of yeast APG8, to a membrane bound form. It is also able to directly interact either with ATG5 or ATG7.
Produced in:	NZ white rabbit
Purity:	Whole serum
Applications:	IHC, immunofluorescence. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. The optimal dilution should be determined by the end user.
Specificity:	IHC, WB and ELISA confirmed the specificity for APG10.
Cross-react:	Human. Other species not yet tested.
Form:	Lyophilised
Reconstitution:	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage:	After reconstitution keep aliquots at minus 20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date:	Six months after purchase
References:	1. Wan D. et al. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729 (2004) 2. Ota T. et al. Nat. Genet. 36:40-45(2004) 3. The MGC Project Team. Genome Res. 14:2121-2127(2004).
Related items:	R-137-500, R-112-100, R-138-500, R-111-100, R-158-100, R-144-100, R-159-100, R-157-100, R-156-100, R-161-100, R-160-100, R-141-100, R-142-100, R-143-100, R-146-100, R-155-100, R-140-100

Images

Confocal microscopy on immunofluorescently detected APG10 in cytospin-isolated human white blood cells using Rabbit antibody to APG10: whole serum (R-145-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for APG10 appear in red. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
More infromation on the staining:
- Fixation: In acetone for 90 seconds
- Antibody dilutions: Primary antibody was diluted 1:100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1:100 alexa 546 conjugate) was incubated for 30 minutes.
- Magnification 80X

PDF Data Sheet	- Click to download the Data Sheet
MSDS	- Click to download the Material Safety Data Sheet

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