| Catalogue No.: |
R-145-100 |
| Batch No.: |
See product label |
| Unit size: |
100 µl |
| Antigen: |
A peptide (CLKNSQKINKNV) corresponding to the C-terminal of human APG10 protein has been used as the immunogen. |
| Other Names: |
Autophagy-related protein 10, APG10-like, GENE NAME: ATG10 |
| Accession: |
APG10_HUMAN |
| Description: |
APG10 plays a role in autophagy. It is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: APG12-APG5 conjugations and modificatioon of a soluble form of MAPLC3 1A, a homolog of yeast APG8, to a membrane bound form. It is also able to directly interact either with ATG5 or ATG7. |
| Produced in: |
NZ white rabbit |
| Purity: |
Whole serum |
| Applications: |
IHC, immunofluorescence. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. The optimal dilution should be determined by the end user. |
| Specificity: |
IHC, WB and ELISA confirmed the specificity for APG10. |
| Cross-reactivity: |
Human. Other species not yet tested. |
| Form: |
Lyophilised |
| Reconstitution: |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage: |
After reconstitution keep aliquots at minus 20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date: |
Six months after purchase |
| References: |
1. Wan D. et al. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729 (2004)
2. Ota T. et al. Nat. Genet. 36:40-45(2004)
3. The MGC Project Team. Genome Res. 14:2121-2127(2004). |
