| Catalogue No.: |
R-137-500 |
| Batch No.: |
See product label |
| Unit size: |
500 µg |
| Antigen: |
A synthetic peptide corresponding to the N-terminal of human APG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response. |
| Other Names: |
Autophagy-related protein 12, APG12-like. GENE NAME: ATG12; APG12; APG12L |
| Accession: |
ATG12_HUMAN |
| Description: |
FUNCTION: Required for autophagy. SUBUNIT: Conjugated to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the ATG12 family. |
| Produced in: |
NZ white rabbit |
| Purity: |
Protein G purified IgG |
| Applications: |
IHC, immunofluorescence, WB. A working concentration of 1-5 µg/ml is recommended. The optimal dilution should be determined by the end user. |
| Specificity: |
IHC and wb confirmed the specificity for APG12. |
| Cross-reactivity: |
Human, rat |
| Form: |
Lyophilised |
| Reconstitution: |
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage: |
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date: |
12 months after purchase |
| References: |
1. Mizushima, N. et al. J. Biol. Chem. 273(51):33889-33892 (1998).
2. Tanida, I. et al. Biochem. Biophys. Res. Commun. 296(5):1164-1170 (2002).
3. Tanida, I. et al. J. Biol. Chem. 277(16):13739-13744 (2002). |
